Difference between revisions of "Part:BBa K1587008:Design"
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===Source=== | ===Source=== | ||
− | Sequences | + | Sequences from cyanobacteria ''Synechocystis sp.'' PCC 6803 :<br> |
- Cph1 domain<br> | - Cph1 domain<br> | ||
− | - Ho1 BBa_I15008<br> | + | - Ho1 [https://parts.igem.org/Part:BBa_I15008 BBa_I15008]<br> |
− | - PcyA BBa_I15009<br> | + | - PcyA [https://parts.igem.org/Part:BBa_I15009 BBa_I15009]<br> |
+ | |||
+ | envZ is a sequence from ''Escherichia coli''. | ||
===References=== | ===References=== | ||
− | Gambetta GA & Lagarias JC (2001) Genetic engineering of phytochrome biosynthesis in bacteria. PNAS 98: 10566–10571 | + | - Gambetta GA & Lagarias JC (2001) Genetic engineering of phytochrome biosynthesis in bacteria. PNAS 98: 10566–10571 |
− | Levskaya A, Chevalier AA, Tabor JJ, Simpson ZB, Lavery LA, Levy M, Davidson EA, Scouras A, Ellington AD, Marcotte EM & Voigt CA (2005) Synthetic biology: Engineering Escherichia coli to see light. Nature 438: 441–442 | + | - Levskaya A, Chevalier AA, Tabor JJ, Simpson ZB, Lavery LA, Levy M, Davidson EA, Scouras A, Ellington AD, Marcotte EM & Voigt CA (2005) Synthetic biology: Engineering Escherichia coli to see light. Nature 438: 441–442 |
− | Lee JM, Lee J, Kim T & Lee SK (2013) Switchable gene expression in Escherichia coli using a miniaturized photobioreactor. PLoS ONE 8: e52382 | + | - Lee JM, Lee J, Kim T & Lee SK (2013) Switchable gene expression in Escherichia coli using a miniaturized photobioreactor. PLoS ONE 8: e52382 |
Latest revision as of 21:11, 18 September 2015
Red light biosensor
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 414
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
No information
Source
Sequences from cyanobacteria Synechocystis sp. PCC 6803 :
- Cph1 domain
- Ho1 BBa_I15008
- PcyA BBa_I15009
envZ is a sequence from Escherichia coli.
References
- Gambetta GA & Lagarias JC (2001) Genetic engineering of phytochrome biosynthesis in bacteria. PNAS 98: 10566–10571
- Levskaya A, Chevalier AA, Tabor JJ, Simpson ZB, Lavery LA, Levy M, Davidson EA, Scouras A, Ellington AD, Marcotte EM & Voigt CA (2005) Synthetic biology: Engineering Escherichia coli to see light. Nature 438: 441–442
- Lee JM, Lee J, Kim T & Lee SK (2013) Switchable gene expression in Escherichia coli using a miniaturized photobioreactor. PLoS ONE 8: e52382