Difference between revisions of "Part:BBa K1713000:Design"
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===Design Notes===
 
===Design Notes===
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The Plac/ara-1 is a hybrid promoter which is composed of the activation operator site from the araBAD promoter and repression operator sites from the lacZYA promoter. And the araBAD promoter is placed in its normal location relative to the transcription start site while the lacZYA promoter is placed both upstream and immediately downstream of the transcription start site. In the meanwhile, It can be activated by the AraC protein in the presence of arabinose and repressed by the LacI protein in the absence of isopropyl b-D-1-thiogalactopyranoside (IPTG).
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Compared with BBa_K094120, We have 1 site mutation and 1 gap which lead to two more restriction enzyme cutting sites, HindIII and BamHI.
  
 
===Source===
 
===Source===
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PCR
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It was reconstructed through PCR with BBa_K094120 served as template and a pair of primers with two mutation.
  
 
===References===
 
===References===
 
Lutz R, Bujard H. Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements[J]. Nucleic acids research, 1997, 25(6): 1203-1210.
 
Lutz R, Bujard H. Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements[J]. Nucleic acids research, 1997, 25(6): 1203-1210.

Latest revision as of 16:07, 18 September 2015

Plac/ara-1, a hybrid promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 28
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Compared with BBa_K094120, We have 1 site mutation and 1 gap which lead to two more restriction enzyme cutting sites, HindIII and BamHI.

Source

It was reconstructed through PCR with BBa_K094120 served as template and a pair of primers with two mutation.

References

Lutz R, Bujard H. Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements[J]. Nucleic acids research, 1997, 25(6): 1203-1210.