Difference between revisions of "Part:BBa K1593998"
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<partinfo>BBa_K1593998 short</partinfo> | <partinfo>BBa_K1593998 short</partinfo> | ||
| − | this part is uesd for coding cheZ,a chemotactic protein. | + | this part is uesd for coding cheZ, a chemotactic protein. When cheZ is overexpressed, the mobility of bacteria will be improved. |
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| + | == Chemotaxis == | ||
| + | When presence of chemicals, cheA is free from cheW interaction, and cheZ will efficiently remove phosphate group from cheY. | ||
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| + | [[File:4903.jpg]] | ||
== Stracture == | == Stracture == | ||
Latest revision as of 15:51, 18 September 2015
overexpress cheZ
this part is uesd for coding cheZ, a chemotactic protein. When cheZ is overexpressed, the mobility of bacteria will be improved.
Chemotaxis
When presence of chemicals, cheA is free from cheW interaction, and cheZ will efficiently remove phosphate group from cheY.
Stracture
PDB code:1KMI
Growth characteristics
We detected OD600(nm) to identify the effect of cheZ expression on E. coli through time.
The exogenous protein expression affects the growth of bacteria, which was indicated by slight slower grow-up in E. coli containing cheZ plasmid. In total, cheZ doesn't have obvious negative impact on bacteria grown.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 373
Illegal NgoMIV site found at 508 - 1000COMPATIBLE WITH RFC[1000]