Difference between revisions of "Part:BBa K1587003"
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<partinfo>BBa_K1587003 short</partinfo> | <partinfo>BBa_K1587003 short</partinfo> | ||
− | Coding region for the 3-hydroxybutyryl-CoA dehydratase (Crt), an enzyme involved in the butyrate biosynthesis pathway of ''Clostridium acetobutylicum''. This substrate of this enzyme is 3-hydroxybutyryl-coA and the product is Crotonyl-CoA. | + | Coding region for the 3-hydroxybutyryl-CoA dehydratase (Crt), an enzyme involved in the butyrate biosynthesis pathway of ''Clostridium acetobutylicum''. This substrate of this enzyme is 3-hydroxybutyryl-coA and the product is Crotonyl-CoA (Figure 1). |
<center>[[File:TLSE crt.jpg]]</center> | <center>[[File:TLSE crt.jpg]]</center> | ||
<center>'''Figure 1: Crotonyl-CoA formation catalyzed by Crt'''</center> | <center>'''Figure 1: Crotonyl-CoA formation catalyzed by Crt'''</center> | ||
− | We used BBa_K1587003 with other genes to produce butyrate in Escherichia coli (Figure 2). | + | We used [https://parts.igem.org/Part:BBa_K1587003 BBa_K1587003] with other genes to produce butyrate in ''Escherichia coli'' (Figure 2). |
<center>[[File:TLSE ButMetabo.jpg]]</center> | <center>[[File:TLSE ButMetabo.jpg]]</center> |
Latest revision as of 20:40, 18 September 2015
3-hydroxybutyryl-CoA dehydratase
Coding region for the 3-hydroxybutyryl-CoA dehydratase (Crt), an enzyme involved in the butyrate biosynthesis pathway of Clostridium acetobutylicum. This substrate of this enzyme is 3-hydroxybutyryl-coA and the product is Crotonyl-CoA (Figure 1).
We used BBa_K1587003 with other genes to produce butyrate in Escherichia coli (Figure 2).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 609
- 1000COMPATIBLE WITH RFC[1000]