Difference between revisions of "Part:BBa K1593998"

Latest revision as of 15:51, 18 September 2015

overexpress cheZ

this part is uesd for coding cheZ, a chemotactic protein. When cheZ is overexpressed, the mobility of bacteria will be improved.

Chemotaxis

When presence of chemicals, cheA is free from cheW interaction, and cheZ will efficiently remove phosphate group from cheY.

Stracture

PDB code:1KMI

Growth characteristics

We detected OD600(nm) to identify the effect of cheZ expression on E. coli through time.

The exogenous protein expression affects the growth of bacteria, which was indicated by slight slower grow-up in E. coli containing cheZ plasmid. In total, cheZ doesn't have obvious negative impact on bacteria grown.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 373
Illegal NgoMIV site found at 508
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1593998 short</partinfo>
-this part is uesd for coding cheZ,a chemotactic protein.
+this part is uesd for coding cheZ, a chemotactic protein. When cheZ is overexpressed, the mobility of bacteria will be improved.
+== Chemotaxis ==
+When presence of chemicals, cheA is free from cheW interaction, and cheZ will efficiently remove phosphate group from cheY.
+[[File:4903.jpg]]
 == Stracture ==
@@ Line 9: / Line 14: @@
 [[File:4901.jpg]]
+== Growth characteristics ==
+We detected OD600(nm) to identify the effect of cheZ expression on E. coli through time.
+[[File:4902.jpg]]
+The exogenous protein expression affects the growth of bacteria, which was indicated by slight slower grow-up in E. coli containing cheZ plasmid. In total, cheZ doesn't have obvious negative impact on bacteria grown.