Difference between revisions of "Part:BBa K1593210"
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Using T7 strong promoter to express OprF, derived from Pseudomonus areuginosa, would strongly improve the bacterial permeability. | Using T7 strong promoter to express OprF, derived from Pseudomonus areuginosa, would strongly improve the bacterial permeability. | ||
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== T7 == | == T7 == | ||
| − | Information of T7:see | + | Information of T7:see [[Part:BBa_I712074 | BBa_I712074]] |
== OprF == | == OprF == | ||
| − | Informtion of OprF:see | + | Informtion of OprF:see [[Part:BBa_K1593209 | BBa_K1593209]] |
| − | + | ||
== Growth Characterization == | == Growth Characterization == | ||
This is in order to demonstrate that the overexpression of porin won't severely affect the growth of bacteria, at least it won't significantly inhibit bacteria growth and become as kill switch. | This is in order to demonstrate that the overexpression of porin won't severely affect the growth of bacteria, at least it won't significantly inhibit bacteria growth and become as kill switch. | ||
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| + | [[File:4900.jpg]] | ||
| + | == ONPG Assay == | ||
| + | This is to show the absorption capability of the bacteria is improved. | ||
| + | |||
| + | [[File:Ustc-onpg55.png]] | ||
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| + | [[File:Ustc-onpg88.png]] | ||
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| + | <!-- | ||
| + | ===Usage and Biology=== | ||
Latest revision as of 14:56, 18 September 2015
T7-OprF
Using T7 strong promoter to express OprF, derived from Pseudomonus areuginosa, would strongly improve the bacterial permeability.
T7
Information of T7:see BBa_I712074
OprF
Informtion of OprF:see BBa_K1593209
Growth Characterization
This is in order to demonstrate that the overexpression of porin won't severely affect the growth of bacteria, at least it won't significantly inhibit bacteria growth and become as kill switch.
ONPG Assay
This is to show the absorption capability of the bacteria is improved.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 654
- 1000COMPATIBLE WITH RFC[1000]