Difference between revisions of "Part:BBa K1593207"

Latest revision as of 15:12, 18 September 2015

micF Promoter, Sensing Antibiotics

This is a promoter sensing sulfoamide which is previously selected from by Sahar Melamed and Shimshon Belkin

Usage and Biology

Description

micF, an antisense ncRNA targeting ompF RNA, main endogenous porin of Escherichia coli, participating in post-transcriptionally regulation of this outer membrane protein F(ompF). micF is also regarded as one of the promoter of soxS-regulated gene.

RNA Duplex

micF was originally tested by Sahar Melamed and Shimshon Belkin to research the antibiotic resistance of bacteria and features of selected sensing promoters. Sensitivity and specificity of micF was presented, showing its relatively specific signal response when treated with:

sulfamethoxazole-255.2;

sulfadimethoxine-44.9;

colistin-36.9.

The maximal induction of micF strains tested in the course of 10h of exposure.

Test Result

Application

In accordance of micF being responsive to the target compound, we implement micF to sense the existence of sulfamethoxazole and sulfadimethoxine in watersample.

Sensing circuit with micF

Experimental Result

micF and soxS, these two antibiotic substance responding promoters were extracted from E. coli Top 10(K-12 strain) by conducting polymerase chain reaction on its genome.

Electrophoretogram of micF(left)

Reference

A bacterial reporter panel for the detection and classification of antibiotic substances Microbial Biotechnology (2012) 5(4), 536–548 Sahar Melamed, Shimshon Belkin MicF: An Antisense RNA Gene Involved in Response of Escherichia coli to Global Stress Factors 2001 JMB doi:10.1006/jmbi.2001.5029 Sequence and Features BBa_K1593207 SequenceAndFeatures

@@ Line 5: / Line 5: @@
 <!-- Add more about the biology of this part here
+<!-- -->
 ===Usage and Biology===
 ==== Description====
 micF, an antisense ncRNA targeting ompF RNA, main endogenous porin of Escherichia coli, participating in post-transcriptionally regulation of this outer membrane protein F(ompF). micF is also regarded as one of the promoter of soxS-regulated gene.
-[[Image:Antisense_ncRNA_with_ompF_mRNA.png|thumb|center|500px|Calibration curve of concentration of GFP against fluorescence. The conversion factor is 79.429.]]
+[[Image: Antisense_ncRNA_with_ompF_mRNA.png|thumb|none|500px|RNA Duplex]]
-[[Image:E0040 invitro degradation.png|thumb|left|500px|Degradation curve of different GFP concentrations.]]
+<br>
+micF was originally tested by Sahar Melamed and Shimshon Belkin to research the antibiotic resistance of bacteria and features of selected sensing promoters. Sensitivity and specificity of micF was presented, showing its relatively specific signal response when treated with:
-<!-- -->
+*'''sulfamethoxazole'''-255.2;
+*'''sulfadimethoxine'''-44.9;
+*'''colistin'''-36.9.
+The maximal induction of micF strains tested in the course of 10h of exposure.
+[[Image: Promoter_Selection_with_Fluroescence_Intensity.png|thumb|none|500px|Test Result]]
+====Application====
+In accordance of micF being responsive to the target compound, we implement micF to sense the existence of sulfamethoxazole and sulfadimethoxine in watersample.
+[[Image: Micf.png|thumb|none|500px|Sensing circuit with micF]]
+====Experimental Result====
+micF and soxS, these two antibiotic substance responding promoters were extracted from E. coli Top 10(K-12 strain) by conducting polymerase chain reaction on its genome.
+[[Image:MicF_and_SoxS.png|thumb|none|500px| Electrophoretogram of micF(left) ]]
+====Reference====
+<html><i>A bacterial reporter panel for the detection and classification of antibiotic substances <b>Microbial Biotechnology (2012) 5(4), 536–548 </b> Sahar Melamed, Shimshon Belkin</i></html>
+<html><i>MicF: An Antisense RNA Gene Involved in Response of Escherichia coli to Global Stress Factors <b>2001 JMB</b> doi:10.1006/jmbi.2001.5029</i><html>
 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K1593207 SequenceAndFeatures</partinfo>