Difference between revisions of "Part:BBa K1593210"

Latest revision as of 14:56, 18 September 2015

T7-OprF

Using T7 strong promoter to express OprF, derived from Pseudomonus areuginosa, would strongly improve the bacterial permeability.

T7

Information of T7:see BBa_I712074

OprF

Informtion of OprF:see BBa_K1593209

Growth Characterization

This is in order to demonstrate that the overexpression of porin won't severely affect the growth of bacteria, at least it won't significantly inhibit bacteria growth and become as kill switch.

ONPG Assay

This is to show the absorption capability of the bacteria is improved.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 654
1000
COMPATIBLE WITH RFC[1000]

@@ Line 6: / Line 6: @@
+== T7 ==
+Information of T7:see [[Part:BBa_I712074 | BBa_I712074]]
-<!-- Add more about the biology of this part here
+== OprF ==
+Informtion of OprF:see [[Part:BBa_K1593209 | BBa_K1593209]]
+== Growth Characterization ==
+This is in order to demonstrate that the overexpression of porin won't severely affect the growth of bacteria, at least it won't significantly inhibit bacteria growth and become as kill switch.
+[[File:4900.jpg]]
+== ONPG Assay ==
+This is to show the absorption capability of the bacteria is improved.
+[[File:Ustc-onpg55.png]]
+[[File:Ustc-onpg88.png]]
+<!--
 ===Usage and Biology===
-== T7 ==
-Information of T7:see <html><a href="https://parts.igem.org/Part:BBa_I712074" target="_blank">BBa_I712074</a>
-<!--
+<!-- -->
 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K1593210 SequenceAndFeatures</partinfo>
-<!--
+<!-- Uncomment this to enable Functional Parameter display
 ===Functional Parameters===
 <partinfo>BBa_K1593210 parameters</partinfo>
 <!-- -->