Difference between revisions of "Part:BBa K1736200:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | Four ORFs: etnA, etnB, etnC, etn D, codon-optimised for expression in ''Pseudomonas'', each ORF has native RBS upstream | + | Four ORFs: etnA, etnB, etnC, etn D, codon-optimised for expression in ''Pseudomonas putida'', each ORF has native RBS upstream |
===Source=== | ===Source=== | ||
| − | Harmonised sequence (via codon harmonisation algorithm) of the Mycobacterium | + | Harmonised sequence (via codon harmonisation algorithm [http://2015.igem.org/Team:Sydney_Australia/TransOpt TransOpt]) of the ''Mycobacterium smegmatis'' ethene monooxygenase enzyme for expression in ''Pseudomonas''. |
===References=== | ===References=== | ||
Latest revision as of 14:24, 18 September 2015
Harmonised Ethene Monooxygenase
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 554
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 119
Illegal BglII site found at 3698
Illegal BamHI site found at 254
Illegal BamHI site found at 969
Illegal BamHI site found at 1887 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 146
Illegal NgoMIV site found at 1717
Illegal NgoMIV site found at 2286
Illegal NgoMIV site found at 2825
Illegal NgoMIV site found at 3246
Illegal AgeI site found at 3949 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Four ORFs: etnA, etnB, etnC, etn D, codon-optimised for expression in Pseudomonas putida, each ORF has native RBS upstream
Source
Harmonised sequence (via codon harmonisation algorithm [http://2015.igem.org/Team:Sydney_Australia/TransOpt TransOpt]) of the Mycobacterium smegmatis ethene monooxygenase enzyme for expression in Pseudomonas.