Difference between revisions of "Part:BBa K1736000:Design"

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===Design Notes===
 
===Design Notes===
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The BsFP protein was harmonised by the method outlined in Spencer ''et al.'' <sup>1</sup> Note that the part also contains an ''B.subtilis'' RBS.
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The BsFP protein was harmonised by the method outlined in Angov ''et al.'' <sup>1</sup> for which we utilised translation rate quantisations proposed by Spencer ''et al.'' <sup>2</sup>. To download and obtain instructions on how to use the algorithm to harmonise your sequence, refer to the Sydney 2015 [http://2015.igem.org/Team:Sydney_Australia/Modeling modelling page]. Note that the part also contains an ''B.subtilis'' RBS.
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<sup>1</sup> Spencer P et al., 2012, “Silent Substitutions Predictably Alter Translation Elongation Rates and Protein Folding Efficiencies”, Vol 422, pp. 328-335.
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===Source===
 
===Source===
  
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Bacillus Subtilits
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''Bacillus Subtilits''
  
 
===References===
 
===References===
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<sup>1</sup> Angov E et al., 2008, “Heterologous Protein Expression Is Enhanced by Harmonizing the Codon Usage Frequencies of the Target Gene with those of the Expression Host”, PLoS One, Vol 3.
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<sup>2</sup> Spencer P et al., 2012, “Silent Substitutions Predictably Alter Translation Elongation Rates and Protein Folding Efficiencies”, Vol 422, pp. 328-335.

Latest revision as of 13:02, 18 September 2015


Harmonised B. subtilis Falvin Binding Fluorescent Protein (BsFP)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 109
    Illegal BamHI site found at 229
    Illegal XhoI site found at 425
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The BsFP protein was harmonised by the method outlined in Angov et al. 1 for which we utilised translation rate quantisations proposed by Spencer et al. 2. To download and obtain instructions on how to use the algorithm to harmonise your sequence, refer to the Sydney 2015 [http://2015.igem.org/Team:Sydney_Australia/Modeling modelling page]. Note that the part also contains an B.subtilis RBS.

Source

Bacillus Subtilits

References

1 Angov E et al., 2008, “Heterologous Protein Expression Is Enhanced by Harmonizing the Codon Usage Frequencies of the Target Gene with those of the Expression Host”, PLoS One, Vol 3.

2 Spencer P et al., 2012, “Silent Substitutions Predictably Alter Translation Elongation Rates and Protein Folding Efficiencies”, Vol 422, pp. 328-335.