Difference between revisions of "Part:BBa K1807000"
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<partinfo>BBa_K1807000 short</partinfo> | <partinfo>BBa_K1807000 short</partinfo> | ||
| − | This device allows for the IPTG-inducible expression of lacZα peptide which in the presence of | + | This device allows for the IPTG-inducible expression of lacZα peptide which in the presence of Xgal in the medium and chromosomal lacZΔM15 produces blue-coloured colonies. The device was used by iGEM York 2015 as an expression vector. SmaI restriction sites flank the lacZ alpha coding sequence. Cutting the part with this enzyme allows for the in-frame insertion of any desired protein coding sequence that contains the '''Splice-In Flanking Sequences''' (they can be found in the design section). |
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Latest revision as of 16:09, 18 September 2015
Protein generator device suitable for blue-white screening and Gibson Assembly.
This device allows for the IPTG-inducible expression of lacZα peptide which in the presence of Xgal in the medium and chromosomal lacZΔM15 produces blue-coloured colonies. The device was used by iGEM York 2015 as an expression vector. SmaI restriction sites flank the lacZ alpha coding sequence. Cutting the part with this enzyme allows for the in-frame insertion of any desired protein coding sequence that contains the Splice-In Flanking Sequences (they can be found in the design section).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]