Difference between revisions of "Part:BBa K1807000"

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This is iGEM York's BioBrick. TBA
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__NOTOC__
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<partinfo>BBa_K1807000 short</partinfo>
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This device allows for the IPTG-inducible expression of lacZα peptide which in the presence of Xgal in the medium and chromosomal lacZΔM15 produces blue-coloured colonies. The device was used by iGEM York 2015 as an expression vector. SmaI restriction sites flank the lacZ alpha coding sequence. Cutting the part with this enzyme allows for the in-frame insertion of any desired protein coding sequence that contains the '''Splice-In Flanking Sequences''' (they can be found in the design section).
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K1807000 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K1807000 parameters</partinfo>
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Latest revision as of 16:09, 18 September 2015

Protein generator device suitable for blue-white screening and Gibson Assembly.

This device allows for the IPTG-inducible expression of lacZα peptide which in the presence of Xgal in the medium and chromosomal lacZΔM15 produces blue-coloured colonies. The device was used by iGEM York 2015 as an expression vector. SmaI restriction sites flank the lacZ alpha coding sequence. Cutting the part with this enzyme allows for the in-frame insertion of any desired protein coding sequence that contains the Splice-In Flanking Sequences (they can be found in the design section).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]