Difference between revisions of "Part:BBa K1734006"
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This part has been codon optimized for Spodoptera frugiperda (Sf9) | This part has been codon optimized for Spodoptera frugiperda (Sf9) | ||
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| + | https://static.igem.org/mediawiki/2015/1/17/Tec_Monterrey_Conf_His.jpg | ||
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| + | This part has been confirmed by restriction analysis of the pSB1C3 vector | ||
Latest revision as of 10:49, 18 September 2015
6xHis-T2A-Nanoluc
This part encodes a sequence capable to add a purification tag (6X His) at the 3’ terminus of a desired protein. Furthermore, by using the reporter gene (nanoluc) it’s possible to quantify indirectly the protein concentration. The T2A sequence cleaves the protein [1], producing the protein of interest tagged with HisTag and nanoluc separately This part has been codon optimized for Spodoptera frugiperda (Sf9)
This part has been confirmed by restriction analysis of the pSB1C3 vector
Reference:
[1] Kim JH, Lee S-R, Li L-H, Park H-J, Park J-H, Lee KY, et al. (2011) High Cleavage Efficiency of a 2A Peptide Derived from Porcine Teschovirus-1 in Human Cell Lines, Zebrafish and Mice. PLoS ONE 6(4): e18556. doi:10.1371/journal.pone.0018556
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 29
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 532