Difference between revisions of "Part:BBa K1813032"
 
(2 intermediate revisions by 2 users not shown)
Line 2: Line 2:
 
<partinfo>BBa_K1813032 short</partinfo>
 
<partinfo>BBa_K1813032 short</partinfo>
  
−
A construct used to achieve the simultaneous bacterial expression of HUMCYPDB1 (<html><b><a href="https://parts.igem.org/Part:BBa_K1813005">BBa_K1813005</b></a></html>) and the <i> A. gambiae </i> NADPH p450 reductase (<html><b><a href="https://parts.igem.org/Part:BBa_K1813010">BBa_K1813010</b></a></html>) under regulative authority of the LacI repressor. <br>
+
A construct used to achieve the simultaneous bacterial expression of CYP2D6 (<html><b><a href="https://parts.igem.org/Part:BBa_K1813005">BBa_K1813005</b></a></html>) and the <i> A. gambiae </i> NADPH P450 reductase (<html><b><a href="https://parts.igem.org/Part:BBa_K1813010">BBa_K1813010</b></a></html>) under regulative authority of the LacI repressor. <br>
  
  
Line 12: Line 12:
 
<!-- -->
 
<!-- -->
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
−
<partinfo>BBa_K1813024 SequenceAndFeatures</partinfo>
+
<partinfo>BBa_K1813032 SequenceAndFeatures</partinfo>
  
  
Line 20: Line 20:
 
<!-- -->
 
<!-- -->
 
<h2> Background </h2>
 
<h2> Background </h2>
−
Repressed by the presence of the LacI regulatory element, this construct does not allow significant expression of either gene without induction by the presence of Isopropyl β-D-1-thiogalactopyranoside in a bacterial host. In doing so, the cytochrome p450 enzyme receives a steady supply of electrons from the reductase, critical for p450 mediated catalysis, once the construct is expressed.
+
Repressed by the presence of the LacI regulatory element, this construct does not allow significant expression of either gene without induction by the presence of Isopropyl β-D-1-thiogalactopyranoside in a bacterial host. In doing so, the cytochrome p450 enzyme receives a steady supply of electrons from the reductase, critical for P450 mediated catalysis, once the construct is expressed.

Latest revision as of 00:39, 19 September 2015

LacIR, AgCPR and CYP2D6 Regulon

A construct used to achieve the simultaneous bacterial expression of CYP2D6 (BBa_K1813005) and the A. gambiae NADPH P450 reductase (BBa_K1813010) under regulative authority of the LacI repressor.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 105
    Illegal BglII site found at 3978
    Illegal BamHI site found at 1887
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4568
    Illegal AgeI site found at 1685
    Illegal AgeI site found at 2867
    Illegal AgeI site found at 3023
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3383


Background

Repressed by the presence of the LacI regulatory element, this construct does not allow significant expression of either gene without induction by the presence of Isopropyl β-D-1-thiogalactopyranoside in a bacterial host. In doing so, the cytochrome p450 enzyme receives a steady supply of electrons from the reductase, critical for P450 mediated catalysis, once the construct is expressed.