Difference between revisions of "Part:BBa K1813024"
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<partinfo>BBa_K1813024 short</partinfo> | <partinfo>BBa_K1813024 short</partinfo> | ||
− | A construct used to achieve the bacterial expression of | + | A construct used to achieve the bacterial expression of CYP2D6 (<html><b><a href="https://parts.igem.org/Part:BBa_K1813005">BBa_K1813005</b></a></html>) under regulative authority of the LacI repressor. |
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<h2> Background </h2> | <h2> Background </h2> | ||
− | Repressed by the presence of the LacI regulatory element, this construct does not allow significant expression of | + | Repressed by the presence of the LacI regulatory element, this construct does not allow significant expression of CYP2D6 without induction by the presence of Isopropyl β-D-1-thiogalactopyranoside in a bacterial host. |
Latest revision as of 00:31, 19 September 2015
CYP2D6 Expression Cassette with LacI Reversed
A construct used to achieve the bacterial expression of CYP2D6 (BBa_K1813005) under regulative authority of the LacI repressor.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 105
Illegal BglII site found at 1737 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2327
- 1000COMPATIBLE WITH RFC[1000]
Background
Repressed by the presence of the LacI regulatory element, this construct does not allow significant expression of CYP2D6 without induction by the presence of Isopropyl β-D-1-thiogalactopyranoside in a bacterial host.