Difference between revisions of "Part:BBa K1769005"

 
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===Usage and Biology===
 
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MtrB, an outer membrane protein from Shewanella oneidensis MR-1, is required for proper incorporation of cytochromes into the outer membrane. This protein is important in stablizing OcmA and OcmB. two outer membrane protein that plays an essential role in netal reduction pathway in S.oneidensis and is also important in genetraing current in microbialfuel cell. We reintroduce this gene into a mtrB gene knockout mutant Shewanella JG700 to create a sensor with electric current as reporter. With a suitable inducible promoter, mtrB will be activated whenever the promoter is induced.</p>  
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MtrB, an outer membrane protein from Shewanella oneidensis MR-1, is required for proper incorporation of cytochromes into the outer membrane. This protein is important in stablizing OcmA and OcmB. Two outer membrane protein that plays an essential role in netal reduction pathway in S.oneidensis and is also important in genetraing current in microbialfuel cell. We reintroduce this gene into a mtrB gene knockout mutant Shewanella JG700 to create a sensor with electric current as reporter. With a suitable inducible promoter, mtrB will be activated whenever the promoter is induced.</p>  
  
 
<img src="https://static.igem.org/mediawiki/parts/9/95/Elecmtrb.jpg" style="align:left; width:40%; padding-bottom:2%">
 
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<p>Sequence and Features</p>
===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K1769005 SequenceAndFeatures</partinfo>
 
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Latest revision as of 08:13, 18 September 2015

MtrB Outer Membrane Structural Protein

Usage and Biology

MtrB, an outer membrane protein from Shewanella oneidensis MR-1, is required for proper incorporation of cytochromes into the outer membrane. This protein is important in stablizing OcmA and OcmB. Two outer membrane protein that plays an essential role in netal reduction pathway in S.oneidensis and is also important in genetraing current in microbialfuel cell. We reintroduce this gene into a mtrB gene knockout mutant Shewanella JG700 to create a sensor with electric current as reporter. With a suitable inducible promoter, mtrB will be activated whenever the promoter is induced.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]