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===Results===
 
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In Figure 1 samples composite parts are presented, the first BBa_K1684004, is composed of the chromoprotein sequence optimized for E. coli K12 spisPink (BBa_K1684000) and a terminator (BBa_B0010); the second part is composed of the chromoprotein optimized sequence amilGFP (BBa_K1684001) and the same terminator (BBa_B0010). This agarose gel shown on Line 1 and Line 2, the biobrick BBa_K1684004, in the first case the band run corresponding to its size, 2869 bp; however, on Line 2 the result was not the expected and only contamination was obtained.
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Latest revision as of 08:10, 18 September 2015

spisPink + Terminator

Pink chromoprotein optimized for E.coli K12 with the standar Terminator

Usage and Biology

Chromoproteins are a collection of proteins that express pigments of strong colors. This protein, expressed in Stylophora pistillata, was evolving independently from the rest of coral chromoproteins.

The protein has a maximum absorption of 560 nm. This characteristic confers a blue shift by at least 14 nanometers in comparison to other known coral chromoproteins, inducing the production of pink color rather than purple appearance.

This version with the standar RBS is codon optimized for the expression in E.coli K12.

Results

In Figure 1 samples composite parts are presented, the first BBa_K1684004, is composed of the chromoprotein sequence optimized for E. coli K12 spisPink (BBa_K1684000) and a terminator (BBa_B0010); the second part is composed of the chromoprotein optimized sequence amilGFP (BBa_K1684001) and the same terminator (BBa_B0010). This agarose gel shown on Line 1 and Line 2, the biobrick BBa_K1684004, in the first case the band run corresponding to its size, 2869 bp; however, on Line 2 the result was not the expected and only contamination was obtained.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

Alieva NO, Konzen KA, Field SF, Meleshkevitch EA, Hunt ME, et al. (2008) Diversity and Evolution of Coral Fluorescent Proteins. PLoS ONE 3(7): e2680. doi:10.1371/journal.pone.0002680