Difference between revisions of "Part:BBa K1859001:Experience"
 
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===Applications of BBa_K1859001===
 
===Applications of BBa_K1859001===
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===User Reviews===
 
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<!-- DON'T DELETE --><partinfo>BBa_K1859001 StartReviews</partinfo>
 
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<!-- Template for a user review
 
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{|width='80%' style='border:1px solid gray'
 
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|-
 
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|width='10%'|
 
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<partinfo>BBa_K1859001 AddReview number</partinfo>
 
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<I>Username</I>
 
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|width='60%' valign='top'|
 
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Enter the review inofrmation here.
 
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|};
 
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<!-- End of the user review template -->
 
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<!-- DON'T DELETE --><partinfo>BBa_K1859001 EndReviews</partinfo>
 
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<html>
 
<html>
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<p>
 
<p>
 
<a href= "http://2015.igem.org/Team:Gifu" > [iGEM gifu 2015]
 
<a href= "http://2015.igem.org/Team:Gifu" > [iGEM gifu 2015]
 
  </a> .
 
  </a> .
 
</p>
 
</p>
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<p>
 
<p>
 
We attached this part[BBa_1859001] to 5 'sides of  
 
We attached this part[BBa_1859001] to 5 'sides of  
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  </a> .
 
  </a> .
  
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Then, we coupled each BBa_K1859004 and BBa_K1859006 in 3 'sides.
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Then,
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it was connected to each 5 'sides of
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<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1859004" > [BBa_K1859004]
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</a>
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and  
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<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1859006" > [BBa_K1859006]
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</a>.  
  
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In addition,Lacl,RBS and DT conbined to them.
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In addition,Lacl
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<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_R0010" >[BBa_R0010] </a>
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, RBS
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<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_R0034" >[BBa_B0034] </a>
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and DT
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<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_R0010" >[BBa_B0015] </a>
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was conbined with them, making
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<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1859027" >[BBa_K1859027] </a>
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and
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<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1859025" >[BBa_K1859025] </a>
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.
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<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1859027" >[BBa_K1859027] </a>
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is the generator that express long-chain protein with keeping functionality of the protein.
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<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1859025" >[BBa_K1859025] </a>
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is the generator to improve circular efficiency of mRNA including His-RFP.
  
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When this device was expressed in E.coli, Curcular mRNA including the linker was made.
 
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The efficiency that mRNA is circularized changed by sequence of the mRNA.
 
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We let mRNA of RFP circularize and produced a long-chain protein, but the functionality was lost.
 
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It is a problem to make a long-chain protein with keeping functionality.
 
 
</p>
 
</p>
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</html>
 
</html>
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===User Reviews===
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<!-- DON'T DELETE --><partinfo>BBa_K1859001 StartReviews</partinfo>
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<!-- Template for a user review
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{|width='80%' style='border:1px solid gray'
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|-
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|width='10%'|
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<partinfo>BBa_K1859001 AddReview number</partinfo>
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<I>Username</I>
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|width='60%' valign='top'|
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Enter the review inofrmation here.
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|};
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<!-- End of the user review template -->
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<!-- DON'T DELETE --><partinfo>BBa_K1859001 EndReviews</partinfo>

Latest revision as of 04:54, 25 September 2015

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1859001

[iGEM gifu 2015] .

We attached this part[BBa_1859001] to 5 'sides of His-RFP without stop codon [BBa_K1332002] . Then, it was connected to each 5 'sides of [BBa_K1859004] and [BBa_K1859006] . In addition,Lacl [BBa_R0010] , RBS [BBa_B0034] and DT [BBa_B0015] was conbined with them, making [BBa_K1859027] and [BBa_K1859025] . [BBa_K1859027] is the generator that express long-chain protein with keeping functionality of the protein. [BBa_K1859025] is the generator to improve circular efficiency of mRNA including His-RFP.

User Reviews

UNIQ5a2875a16a4b9e77-partinfo-00000001-QINU UNIQ5a2875a16a4b9e77-partinfo-00000002-QINU