Difference between revisions of "Part:BBa K1806002:Experience"
(→Cloning) |
|||
| (2 intermediate revisions by one other user not shown) | |||
| Line 1: | Line 1: | ||
| − | |||
__NOTOC__ | __NOTOC__ | ||
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
| Line 5: | Line 4: | ||
===Applications of BBa_K1806002=== | ===Applications of BBa_K1806002=== | ||
| + | == Cloning == | ||
| + | The system was ligated with the pSB1C3 cloning vector and then transformated to be verified with Colony PCR. | ||
| + | The RFP present in the iGEM Registry contains the Hind3 enzyme restriction site unlike the RFP sequence present in this part. Hence, the part was cut with the EcoR1 and Hind3 restriction enzymes. | ||
| + | |||
| + | Tagged in the gel runs below as G-Block 4, it can be seen that the gel runs yielded accurate base length. | ||
| + | |||
| + | [[File:AUC_TURKEY_4_colony.png]] [[File:6002-2.png]] | ||
| + | |||
| + | [[File:AUCTURKEY4,2cutcheck.png]] [[File:6002-4.png]] | ||
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 10:12, 22 September 2015
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K1806002
Cloning
The system was ligated with the pSB1C3 cloning vector and then transformated to be verified with Colony PCR. The RFP present in the iGEM Registry contains the Hind3 enzyme restriction site unlike the RFP sequence present in this part. Hence, the part was cut with the EcoR1 and Hind3 restriction enzymes.
Tagged in the gel runs below as G-Block 4, it can be seen that the gel runs yielded accurate base length.
User Reviews
UNIQad8b506fc86d98f4-partinfo-00000000-QINU UNIQad8b506fc86d98f4-partinfo-00000001-QINU