Difference between revisions of "Part:BBa K1766003"
 
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MicF is a non-coding RNA which regulates expression of the ompF (outer membrane porin F) gene in E. coli. MicF binds to the 5’-UTR of ompF mRNA, to prevent translation and promote degradation [1].
 
MicF is a non-coding RNA which regulates expression of the ompF (outer membrane porin F) gene in E. coli. MicF binds to the 5’-UTR of ompF mRNA, to prevent translation and promote degradation [1].
  
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This part is intended to be used together with BBa_M30011 and BBa_K1766002 to form an OmpR regulated composite fluorescence system, BBa_K1766004, by iGEM Stockholm team 2015. Similarly, BBa_K1766005 and BBa_K1766006 were supposed to create BBa_K1766007, together with this part. BBa_K1766007 is an OmpR regulated quorum sensing system.
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This part is intended to be used in an OmpR regulated composite fluorescence system, BBa_K1766004, by iGEM Stockholm team 2015. Similarly, BBa_K1766007 was also aimed to be created together with this part. BBa_K1766007 is an OmpR regulated quorum sensing system.
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[[File:MicFOmpR.png|200px|thumb|left|OmpR controlled micF]]
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
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<partinfo>BBa_K1766003 parameters</partinfo>
 
<partinfo>BBa_K1766003 parameters</partinfo>
 
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'''References'''
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[1] Delihas, N; Forst S (2001). "MicF: an antisense RNA gene involved in response of Escherichia coli to global stress factors". J Mol Biol 313 (1): 1–12

Latest revision as of 09:41, 18 September 2015

OmpR controlled MicF

MicF is a non-coding RNA which regulates expression of the ompF (outer membrane porin F) gene in E. coli. MicF binds to the 5’-UTR of ompF mRNA, to prevent translation and promote degradation [1].

This part is intended to be used in an OmpR regulated composite fluorescence system, BBa_K1766004, by iGEM Stockholm team 2015. Similarly, BBa_K1766007 was also aimed to be created together with this part. BBa_K1766007 is an OmpR regulated quorum sensing system.


OmpR controlled micF







Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[1] Delihas, N; Forst S (2001). "MicF: an antisense RNA gene involved in response of Escherichia coli to global stress factors". J Mol Biol 313 (1): 1–12