Difference between revisions of "Part:BBa K1684003"

 
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Yellow chromoprotein optimized for E.coli K12 with the standar RBS
 
Yellow chromoprotein optimized for E.coli K12 with the standar RBS
  
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===Usage and Biology===
 
===Usage and Biology===
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Chromoproteins are a collection of proteins that express pigments of strong colors. The AmilGFP derivate from Acropora millepora, present a yellow pigment when it’s expressed. Also, the protein has a maximum absorption of 512 nm.
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This version with the standar RBS is codon optimized for the expression in E.coli K12.
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===Results===
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The Figure 1  present different samples of parts used for transformation of competent cells composed by a RBS (BBa_B0034) and the optimized chromoprotein sequence amilGFP (BBa_K1684001 ).
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The agarose gel shown in Line 1, the negative control; Line 2, belongs to the purified DNA of pGLO (5371 bp), was run as a positive control. Line 5 and Line 6 contain duplicate BBa_K1684003 (BBa_B0034 + BBa_K1684001) biobrick; however, a positive result is observed only in Line 5, with size of 2822 bp, while in line 6 only presents contamination, without the presence of any genetic material.
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<img class="responsive-img" src="https://static.igem.org/mediawiki/2015/5/50/GEL1BIOSINT2015.jpeg" width="25%" style="position: center; top:0%;"/>
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1684003 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1684003 SequenceAndFeatures</partinfo>
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===References===
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Alieva NO, Konzen KA, Field SF, Meleshkevitch EA, Hunt ME, et al. (2008) Diversity and Evolution of Coral Fluorescent Proteins. PLoS ONE 3(7): e2680.
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doi:10.1371/journal.pone.0002680
  
  

Latest revision as of 08:09, 18 September 2015

RBS + amilGFP

Yellow chromoprotein optimized for E.coli K12 with the standar RBS

Usage and Biology

Chromoproteins are a collection of proteins that express pigments of strong colors. The AmilGFP derivate from Acropora millepora, present a yellow pigment when it’s expressed. Also, the protein has a maximum absorption of 512 nm.

This version with the standar RBS is codon optimized for the expression in E.coli K12.

Results

The Figure 1 present different samples of parts used for transformation of competent cells composed by a RBS (BBa_B0034) and the optimized chromoprotein sequence amilGFP (BBa_K1684001 ).

The agarose gel shown in Line 1, the negative control; Line 2, belongs to the purified DNA of pGLO (5371 bp), was run as a positive control. Line 5 and Line 6 contain duplicate BBa_K1684003 (BBa_B0034 + BBa_K1684001) biobrick; however, a positive result is observed only in Line 5, with size of 2822 bp, while in line 6 only presents contamination, without the presence of any genetic material.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 630
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

Alieva NO, Konzen KA, Field SF, Meleshkevitch EA, Hunt ME, et al. (2008) Diversity and Evolution of Coral Fluorescent Proteins. PLoS ONE 3(7): e2680. doi:10.1371/journal.pone.0002680