Difference between revisions of "Part:BBa K1585202"
 
(2 intermediate revisions by the same user not shown)
Line 2: Line 2:
 
<partinfo>BBa_K1585202 short</partinfo>
 
<partinfo>BBa_K1585202 short</partinfo>
  
−
6-Phospho-3-hexuloisomerase from Bacillus methanolicus MGA3, codon optimized for E.coli, converts D-Arabino-hex-3-ulose-6-phosphate to D-Fructose-6-phosphate
+
6-Phospho-3-hexuloisomerase from ''Bacillus methanolicus'' MGA3, codon optimized for ''E. coli'', converts D-Arabino-hex-3-ulose-6-phosphate to D-Fructose-6-phosphate
  
  
Line 10: Line 10:
 
|<html> <img src="https://static.igem.org/mediawiki/parts/0/01/Aachen_Reaction_phi.png" width="600px"></html>
 
|<html> <img src="https://static.igem.org/mediawiki/parts/0/01/Aachen_Reaction_phi.png" width="600px"></html>
 
|-
 
|-
−
|'''{{{title|Catalysed reaction by Phi}}}'''<br />D-arabino-hex-3-ulose 6-phosphate → D-fructose 6-phosphate.
+
|'''{{{title|Catalyzed reaction by Phi}}}'''<br />D-arabino-hex-3-ulose 6-phosphate → D-fructose 6-phosphate.
 
|}
 
|}
 
</div>
 
</div>
Line 17: Line 17:
  
  
−
<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
 +
 +
[[File:Aachen 15-08-10 Mdh, Hps, Phi f. Wiki 2.png]]
 +
 +
Mdh, Hps and Phi expressed in a pSB1A30 backbone on SDS-PAGE after IPTG induction with a RFP expression strain as a negative control. All genes expressed in BL21 Gold (DE3), samples taken from 50 ml main culture in shake flasks after 6 h and 21 h after IPTG induction.
 +
  
 
<!-- -->
 
<!-- -->

Latest revision as of 23:18, 18 September 2015

PHI

6-Phospho-3-hexuloisomerase from Bacillus methanolicus MGA3, codon optimized for E. coli, converts D-Arabino-hex-3-ulose-6-phosphate to D-Fructose-6-phosphate


Catalyzed reaction by Phi
D-arabino-hex-3-ulose 6-phosphate → D-fructose 6-phosphate.



Usage and Biology

Aachen 15-08-10 Mdh, Hps, Phi f. Wiki 2.png

Mdh, Hps and Phi expressed in a pSB1A30 backbone on SDS-PAGE after IPTG induction with a RFP expression strain as a negative control. All genes expressed in BL21 Gold (DE3), samples taken from 50 ml main culture in shake flasks after 6 h and 21 h after IPTG induction.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]