Difference between revisions of "Part:BBa K1585202"

Latest revision as of 23:18, 18 September 2015

PHI

6-Phospho-3-hexuloisomerase from Bacillus methanolicus MGA3, codon optimized for E. coli, converts D-Arabino-hex-3-ulose-6-phosphate to D-Fructose-6-phosphate

Catalyzed reaction by Phi
D-arabino-hex-3-ulose 6-phosphate → D-fructose 6-phosphate.

Usage and Biology

Mdh, Hps and Phi expressed in a pSB1A30 backbone on SDS-PAGE after IPTG induction with a RFP expression strain as a negative control. All genes expressed in BL21 Gold (DE3), samples taken from 50 ml main culture in shake flasks after 6 h and 21 h after IPTG induction.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1585202 short</partinfo>
--Phospho-3-hexuloisomerase from Bacillus methanolicus MGA3, codon optimized for E.coli, converts D-Arabino-hex-3-ulose-6-phosphate to D-Fructose-6-phosphate
+-Phospho-3-hexuloisomerase from ''Bacillus methanolicus'' MGA3, codon optimized for ''E. coli'', converts D-Arabino-hex-3-ulose-6-phosphate to D-Fructose-6-phosphate
@@ Line 10: / Line 10: @@
 |<html> <img src="https://static.igem.org/mediawiki/parts/0/01/Aachen_Reaction_phi.png" width="600px"></html>
 |-
-|'''{{{title|Catalysed reaction by Phi}}}'''<br />D-arabino-hex-3-ulose 6-phosphate → D-fructose 6-phosphate.
+|'''{{{title|Catalyzed reaction by Phi}}}'''<br />D-arabino-hex-3-ulose 6-phosphate → D-fructose 6-phosphate.
 |}
 </div>
@@ Line 16: / Line 16: @@
 <html></br></html>
-[[File:Aachen_Reaction_phi.png]]
-D-arabino-hex-3-ulose 6-phosphate → D-fructose 6-phosphate
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+[[File:Aachen 15-08-10 Mdh, Hps, Phi f. Wiki 2.png]]
+Mdh, Hps and Phi expressed in a pSB1A30 backbone on SDS-PAGE after IPTG induction with a RFP expression strain as a negative control. All genes expressed in BL21 Gold (DE3), samples taken from 50 ml main culture in shake flasks after 6 h and 21 h after IPTG induction.
 <!-- -->