Difference between revisions of "Part:BBa K1585211"

Latest revision as of 23:07, 18 September 2015

B0034.HPS

This is the translational unit of 3-hexulose-6-phosphate synthase (Hps) from Bacillus methanolicus MGA3. It is codon optimized for E. coli.

Catalyzed reaction by Hps
Formaldehyde + Ribulose-5-phosphat → D-arabino-hex-3-ulose 6-phosphate.

Usage and Biology

Mdh, Hps and Phi expressed in a pSB1A30 backbone on SDS-PAGE after IPTG induction with a RFP expression strain as a negative control. All genes expressed in BL21 Gold (DE3), samples taken from 50 ml main culture in shake flasks after 6 h and 21 h after IPTG induction.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1585211 short</partinfo>
-This is the translational unit of 3-hexulose-6-phosphate synthase (Hps) from Bacillus methanolicus MGA3. It is codon optimized for E.coli.
+This is the translational unit of 3-hexulose-6-phosphate synthase (Hps) from Bacillus methanolicus MGA3. It is codon optimized for ''E. coli''.
@@ Line 10: / Line 10: @@
 |<html> <img src="https://static.igem.org/mediawiki/parts/b/b8/Aachen_Reaction_hps.png" width="600px"></html>
 |-
-|'''{{{title|Catalysed reaction by Hps}}}'''<br />Formaldehyde + Ribulose-5-phosphat  →  D-arabino-hex-3-ulose 6-phosphate.
+|'''{{{title|Catalyzed reaction by Hps}}}'''<br />Formaldehyde + Ribulose-5-phosphat  →  D-arabino-hex-3-ulose 6-phosphate.
 |}
 </div>
@@ Line 17: / Line 17: @@
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+[[File:Aachen 15-08-10 Mdh, Hps, Phi f. Wiki 2.png]]
+Mdh, Hps and Phi expressed in a pSB1A30 backbone on SDS-PAGE after IPTG induction with a RFP expression strain as a negative control. All genes expressed in BL21 Gold (DE3), samples taken from 50 ml main culture in shake flasks after 6 h and 21 h after IPTG induction.
 <!-- -->