Difference between revisions of "Part:BBa K1587000"
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<partinfo>BBa_K1587000 short</partinfo> | <partinfo>BBa_K1587000 short</partinfo> | ||
− | Strong RBS (BBa_B0030). Heme oxygenase-1 (Ho1) coding region (BBa_K566022). | + | Strong RBS (BBa_B0030). Heme oxygenase-1 (Ho1) coding region ([https://parts.igem.org/Part:BBa_K566022 BBa_K566022]). |
− | ho1, along with pcyA, converts heme into the chromophore phycocyanobillin (PCB). | + | ho1, along with pcyA, converts heme into the chromophore phycocyanobillin (PCB) (Figure 1).<br> |
+ | The integrity of our construct was checked by sequencing using VF2 primer. | ||
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+ | <center>[[File:TLSE pcyA ho1.jpg]]</center> | ||
+ | <center>'''Figure 1: pcyA catalysed formation of PCB from biliverdin (BV) IXα. (Ken et <i>al.</i> 2009)'''</center> | ||
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+ | Ho1 is used to catalyse the formation of biliverdin (BV) IXα via heme. It is usully used in association with PcyA enabeling the formation phycocyanobilin (PCB), a chromophore of the phytochrome family of photoreceptors, photosynthetic light-harvesting complexes required for circadian clock. | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Latest revision as of 20:36, 18 September 2015
ho1 with RBS
Strong RBS (BBa_B0030). Heme oxygenase-1 (Ho1) coding region (BBa_K566022).
ho1, along with pcyA, converts heme into the chromophore phycocyanobillin (PCB) (Figure 1).
The integrity of our construct was checked by sequencing using VF2 primer.
Ho1 is used to catalyse the formation of biliverdin (BV) IXα via heme. It is usully used in association with PcyA enabeling the formation phycocyanobilin (PCB), a chromophore of the phytochrome family of photoreceptors, photosynthetic light-harvesting complexes required for circadian clock.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]