Difference between revisions of "Part:BBa K1585212"

Latest revision as of 23:16, 18 September 2015

B0034.PHI

This is the translational unit of the 6-phospho-3-hexuloisomerase (Phi) from Bacillus methanolicus MGA3. It is codon optimized for E. coli.

Catalysed reaction by Phi
D-arabino-hex-3-ulose 6-phosphate → D-fructose 6-phosphate.

Usage and Biology

Mdh, Hps and Phi expressed in a pSB1A30 backbone on SDS-PAGE after IPTG induction with a RFP expression strain as a negative control. All genes expressed in BL21 Gold (DE3), samples taken from 50 ml main culture in shake flasks after 6 h and 21 h after IPTG induction.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1585212 short</partinfo>
-This is the translational unit of the 6-phospho-3-hexuloisomerase (PHI) from Bacillus methanolicus MGA3. It is codon optimized for E.coli.
+This is the translational unit of the 6-phospho-3-hexuloisomerase (Phi) from ''Bacillus methanolicus'' MGA3. It is codon optimized for ''E. coli''.
-[[File:Aachen_Reaction_phi.png]]
-D-arabino-hex-3-ulose 6-phosphate → D-fructose 6-phosphate
+<center>
+<div class="figure" style="float:{{{align|center}}}; margin: 0px 10px 10px 0px; border:{{{border|0px solid #aaa}}};width:{{{width|600px}}};padding:10px 10px 0px 0px;">
+{|
+|<html> <img src="https://static.igem.org/mediawiki/parts/0/01/Aachen_Reaction_phi.png" width="600px"></html>
+|-
+|'''{{{title|Catalysed reaction by Phi}}}'''<br />D-arabino-hex-3-ulose 6-phosphate → D-fructose 6-phosphate.
+|}
+</div>
+</center>
+<html></br></html>
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+[[File:Aachen 15-08-10 Mdh, Hps, Phi f. Wiki 2.png]]
+Mdh, Hps and Phi expressed in a pSB1A30 backbone on SDS-PAGE after IPTG induction with a RFP expression strain as a negative control. All genes expressed in BL21 Gold (DE3), samples taken from 50 ml main culture in shake flasks after 6 h and 21 h after IPTG induction.
 <!-- -->