Difference between revisions of "Part:BBa K1766002:Design"

 
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===Design Notes===
 
===Design Notes===
This is a second version of a design that was faulty. The design was modified using site directed mutagenesis and therefor contains residues from the faulty design with should not interfere with the new design. For more detail about the making of this part please visit the [http://2015.igem.org/Team:Stockholm/Results 2015 iGEM Stockholm wiki page] or contact [www.l.r.osterberg@gmail.com Linnea Österberg]
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This is a second version of a design that was faulty. The design was modified using site directed mutagenesis. The part contains residues from the faulty design, however, they should not interfere with the new design. For more detail about the making of this part, please visit the [http://2015.igem.org/Team:Stockholm/Results 2015 iGEM Stockholm wiki page] or contact [mailto:l.r.osterberg@gmail.com Linnea Österberg].
 
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===Source===
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''E. coli''
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===References===
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Latest revision as of 23:49, 17 September 2015

MicF regulated GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 861


Design Notes

This is a second version of a design that was faulty. The design was modified using site directed mutagenesis. The part contains residues from the faulty design, however, they should not interfere with the new design. For more detail about the making of this part, please visit the [http://2015.igem.org/Team:Stockholm/Results 2015 iGEM Stockholm wiki page] or contact Linnea Österberg.