Difference between revisions of "Part:BBa K1587007:Design"
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- pflA http://www.ncbi.nlm.nih.gov/nuccore/NC_000913.3?report=fasta&from=950340&to=951080&strand=true | - pflA http://www.ncbi.nlm.nih.gov/nuccore/NC_000913.3?report=fasta&from=950340&to=951080&strand=true | ||
− | The strong RBS and | + | The strong RBS and terminator were used according to respectively BBa_B0030 and BBa_B1006 |
===References=== | ===References=== | ||
- Crain AV, Broderick JB. 2014. Pyruvate Formate-lyase and Its Activation by Pyruvate Formate-lyase Activating Enzyme. J Biol Chem 289:5723–5729. | - Crain AV, Broderick JB. 2014. Pyruvate Formate-lyase and Its Activation by Pyruvate Formate-lyase Activating Enzyme. J Biol Chem 289:5723–5729. | ||
+ | |||
+ | - Wei X-X, Zheng W-T, Hou X, Liang J, Li Z-J, Wei X-X, Zheng W-T, Hou X, Liang J & Li Z-J (2015) Metabolic Engineering of Escherichia coli for Poly(3-hydroxybutyrate) Production under Microaerobic Condition. BioMed Research International, BioMed Research International 2015, 2015: e789315 |
Latest revision as of 16:36, 18 September 2015
Formate production
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 405
Illegal BamHI site found at 2807 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 514
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2995
Design Notes
Source
Both genes are from E. coli :
- pflB http://www.ncbi.nlm.nih.gov/nuccore/NC_000913.3?report=fasta&from=951272&to=953554&strand=true
- pflA http://www.ncbi.nlm.nih.gov/nuccore/NC_000913.3?report=fasta&from=950340&to=951080&strand=true
The strong RBS and terminator were used according to respectively BBa_B0030 and BBa_B1006
References
- Crain AV, Broderick JB. 2014. Pyruvate Formate-lyase and Its Activation by Pyruvate Formate-lyase Activating Enzyme. J Biol Chem 289:5723–5729.
- Wei X-X, Zheng W-T, Hou X, Liang J, Li Z-J, Wei X-X, Zheng W-T, Hou X, Liang J & Li Z-J (2015) Metabolic Engineering of Escherichia coli for Poly(3-hydroxybutyrate) Production under Microaerobic Condition. BioMed Research International, BioMed Research International 2015, 2015: e789315