Difference between revisions of "Part:BBa K1796010"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1796010 short</partinfo>
 
<partinfo>BBa_K1796010 short</partinfo>
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<p>
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Function: scaffold gene of nitrogen fixatiion gene cluster,encode FeMo-cofactor assembly protein.
  
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Function: scaffold
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</p>
  
  
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<partinfo>BBa_K1796010 parameters</partinfo>
 
<partinfo>BBa_K1796010 parameters</partinfo>
 
<!-- -->
 
<!-- -->
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 +
==Parameter of Protein==
 +
<p> Number of amino acids: 452 </p>
 +
<p> </p>
 +
<p> Molecular weight: 49505.3 </p>
 +
<p> </p>
 +
<p> Theoretical pI: 6.01 </p>
 +
<p> </p>
 +
<p> Amino acid composition:  </p>
 +
<p> </p>
 +
<p> Ala (A)  39      8.6% </p>
 +
<p> </p>
 +
<p> Arg (R)  29      6.4% </p>
 +
<p> </p>
 +
<p> Asn (N)  11      2.4% </p>
 +
<p> </p>
 +
<p> Asp (D)  19      4.2% </p>
 +
<p> </p>
 +
<p> Cys (C)  7      1.5% </p>
 +
<p> </p>
 +
<p> Gln (Q)  16      3.5% </p>
 +
<p> </p>
 +
<p> Glu (E)  37      8.2% </p>
 +
<p> </p>
 +
<p> Gly (G)  45      10.0% </p>
 +
<p> </p>
 +
<p> His (H)  10      2.2% </p>
 +
<p> </p>
 +
<p> Ile (I)  30      6.6% </p>
 +
<p> </p>
 +
<p> Leu (L)  38      8.4% </p>
 +
<p> </p>
 +
<p> Lys (K)  21      4.6% </p>
 +
<p> </p>
 +
<p> Met (M)  10      2.2% </p>
 +
<p> </p>
 +
<p> Phe (F)  12      2.7% </p>
 +
<p> </p>
 +
<p> Pro (P)  21      4.6% </p>
 +
<p> </p>
 +
<p> Ser (S)  32      7.1% </p>
 +
<p> </p>
 +
<p> Thr (T)  24      5.3% </p>
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<p> </p>
 +
<p> Trp (W)  6      1.3% </p>
 +
<p> </p>
 +
<p> Tyr (Y)  15      3.3% </p>
 +
<p> </p>
 +
<p> Val (V)  30      6.6% </p>
 +
<p> </p>
 +
<p> Pyl (O)  0      0.0% </p>
 +
<p> </p>
 +
<p> Sec (U)  0      0.0% </p>
 +
<p> </p>
 +
<p> (B)  0          0.0% </p>
 +
<p> </p>
 +
<p> (Z)  0          0.0% </p>
 +
<p> </p>
 +
<p> (X)  0          0.0% </p>
 +
<p> </p>
 +
<p> Total number of negatively charged residues (Asp + Glu): 56 </p>
 +
<p> Total number of positively charged residues (Arg + Lys): 50 </p>
 +
<p> </p>
 +
<p> Atomic composition:Carbon      C              2187 </p>
 +
<p> Hydrogen    H        3471 </p>
 +
<p> Nitrogen    N          613 </p>
 +
<p> Oxygen      O          663 </p>
 +
<p> Sulfur      S          17 </p>
 +
<p> </p>
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<p> Formula: C2187H3471N613O663S17Total number of atoms: 6951 </p>
 +
<p> </p>
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<p> Extinction coefficients:Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water. </p>
 +
<p> </p>
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<p> Ext. coefficient    55725 </p>
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<p> Abs 0.1% (=1 g/l)  1.126, assuming all pairs of Cys residues form cystines </p>
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<p> </p>
 +
<p> </p>
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<p> Ext. coefficient    55350 </p>
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<p> Abs 0.1% (=1 g/l)  1.118, assuming all Cys residues are reduced </p>
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<p> </p>
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<p> Estimated half-life:The N-terminal of the sequence considered is E (Glu). </p>
 +
<p> </p>
 +
<p> The estimated half-life is: 1 hours (mammalian reticulocytes, in vitro). </p>
 +
<p>                             30 min (yeast, in vivo). </p>
 +
<p>                             >10 hours (Escherichia coli, in vivo). </p>
 +
<p> </p>
 +
<p> </p>
 +
<p> Instability index:The instability index (II) is computed to be 40.70 </p>
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<p> This classifies the protein as unstable. </p>
 +
<p> </p>
 +
<p> </p>
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<p> </p>
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<p> Aliphatic index: 86.55 </p>
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<p> </p>
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<p> Grand average of hydropathicity (GRAVY): -0.239 </p>
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<p> </p>
 +
<p> </p>
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<p> </p>

Latest revision as of 02:35, 19 September 2015

nifE promoted from Paenibacillus sp. WLY78

Function: scaffold gene of nitrogen fixatiion gene cluster,encode FeMo-cofactor assembly protein.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1369
    Illegal PstI site found at 1015
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1369
    Illegal PstI site found at 1015
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1369
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1369
    Illegal PstI site found at 1015
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1369
    Illegal PstI site found at 1015
    Illegal NgoMIV site found at 360
    Illegal NgoMIV site found at 607
  • 1000
    COMPATIBLE WITH RFC[1000]


Parameter of Protein

Number of amino acids: 452

Molecular weight: 49505.3

Theoretical pI: 6.01

Amino acid composition: 

Ala (A) 39 8.6%

Arg (R) 29 6.4%

Asn (N) 11 2.4%

Asp (D) 19 4.2%

Cys (C) 7 1.5%

Gln (Q) 16 3.5%

Glu (E) 37 8.2%

Gly (G) 45 10.0%

His (H) 10 2.2%

Ile (I) 30 6.6%

Leu (L) 38 8.4%

Lys (K) 21 4.6%

Met (M) 10 2.2%

Phe (F) 12 2.7%

Pro (P) 21 4.6%

Ser (S) 32 7.1%

Thr (T) 24 5.3%

Trp (W) 6 1.3%

Tyr (Y) 15 3.3%

Val (V) 30 6.6%

Pyl (O) 0 0.0%

Sec (U) 0 0.0%

(B) 0 0.0%

(Z) 0 0.0%

(X) 0 0.0%

Total number of negatively charged residues (Asp + Glu): 56

Total number of positively charged residues (Arg + Lys): 50

Atomic composition:Carbon C 2187

Hydrogen H 3471

Nitrogen N 613

Oxygen O 663

Sulfur S 17

Formula: C2187H3471N613O663S17Total number of atoms: 6951

Extinction coefficients:Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water.

Ext. coefficient 55725

Abs 0.1% (=1 g/l) 1.126, assuming all pairs of Cys residues form cystines

Ext. coefficient 55350

Abs 0.1% (=1 g/l) 1.118, assuming all Cys residues are reduced

Estimated half-life:The N-terminal of the sequence considered is E (Glu).

The estimated half-life is: 1 hours (mammalian reticulocytes, in vitro).

30 min (yeast, in vivo).

>10 hours (Escherichia coli, in vivo).

Instability index:The instability index (II) is computed to be 40.70

This classifies the protein as unstable.

Aliphatic index: 86.55

Grand average of hydropathicity (GRAVY): -0.239