Difference between revisions of "Part:BBa K1796103"

 
 
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__NOTOC__
 
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<partinfo>BBa_K1796103 short</partinfo>
 
<partinfo>BBa_K1796103 short</partinfo>
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<partinfo>BBa_K1796103 parameters</partinfo>
 
<partinfo>BBa_K1796103 parameters</partinfo>
 
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 +
 +
==Parameter of Protein==
 +
<p> Number of amino acids: 681 </p>
 +
<p> </p>
 +
<p> Molecular weight: 79647.4 </p>
 +
<p> </p>
 +
<p> Theoretical pI: 11.06 </p>
 +
<p> </p>
 +
<p> Amino acid composition:  </p>
 +
<p> </p>
 +
<p> Ala (A)  44      6.5% </p>
 +
<p> </p>
 +
<p> Arg (R) 121      17.8% </p>
 +
<p> </p>
 +
<p> Asn (N)  21      3.1% </p>
 +
<p> </p>
 +
<p> Asp (D)  28      4.1% </p>
 +
<p> </p>
 +
<p> Cys (C)  30      4.4% </p>
 +
<p> </p>
 +
<p> Gln (Q)  17      2.5% </p>
 +
<p> </p>
 +
<p> Glu (E)  12      1.8% </p>
 +
<p> </p>
 +
<p> Gly (G)  54      7.9% </p>
 +
<p> </p>
 +
<p> His (H)  38      5.6% </p>
 +
<p> </p>
 +
<p> Ile (I)  12      1.8% </p>
 +
<p> </p>
 +
<p> Leu (L)  59      8.7% </p>
 +
<p> </p>
 +
<p> Lys (K)  10      1.5% </p>
 +
<p> </p>
 +
<p> Met (M)  1      0.1% </p>
 +
<p> </p>
 +
<p> Phe (F)  17      2.5% </p>
 +
<p> </p>
 +
<p> Pro (P)  66      9.7% </p>
 +
<p> </p>
 +
<p> Ser (S)  55      8.1% </p>
 +
<p> </p>
 +
<p> Thr (T)  18      2.6% </p>
 +
<p> </p>
 +
<p> Trp (W)  17      2.5% </p>
 +
<p> </p>
 +
<p> Tyr (Y)  42      6.2% </p>
 +
<p> </p>
 +
<p> Val (V)  19      2.8% </p>
 +
<p> </p>
 +
<p> Pyl (O)  0      0.0% </p>
 +
<p> </p>
 +
<p> Sec (U)  0      0.0% </p>
 +
<p> </p>
 +
<p> (B)  0          0.0% </p>
 +
<p> </p>
 +
<p> (Z)  0          0.0% </p>
 +
<p> </p>
 +
<p> (X)  0          0.0% </p>
 +
<p> </p>
 +
<p> Total number of negatively charged residues (Asp + Glu): 40 </p>
 +
<p> Total number of positively charged residues (Arg + Lys): 131 </p>
 +
<p> </p>
 +
<p> Atomic composition:Carbon      C              3496 </p>
 +
<p> Hydrogen    H        5383 </p>
 +
<p> Nitrogen    N        1185 </p>
 +
<p> Oxygen      O          915 </p>
 +
<p> Sulfur      S          31 </p>
 +
<p> </p>
 +
<p> Formula: C3496H5383N1185O915S31Total number of atoms: 11010 </p>
 +
<p> </p>
 +
<p> Extinction coefficients:Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water. </p>
 +
<p> </p>
 +
<p> Ext. coefficient  157955 </p>
 +
<p> Abs 0.1% (=1 g/l)  1.983, assuming all pairs of Cys residues form cystines </p>
 +
<p> </p>
 +
<p> </p>
 +
<p> Ext. coefficient  156080 </p>
 +
<p> Abs 0.1% (=1 g/l)  1.960, assuming all Cys residues are reduced </p>
 +
<p> </p>
 +
<p> Estimated half-life:The N-terminal of the sequence considered is E (Glu). </p>
 +
<p> </p>
 +
<p> The estimated half-life is: 1 hours (mammalian reticulocytes, in vitro). </p>
 +
<p>                             30 min (yeast, in vivo). </p>
 +
<p>                             >10 hours (Escherichia coli, in vivo). </p>
 +
<p> </p>
 +
<p> </p>
 +
<p> Instability index:The instability index (II) is computed to be 74.60 </p>
 +
<p> This classifies the protein as unstable. </p>
 +
<p> </p>
 +
<p> </p>
 +
<p> </p>
 +
<p> Aliphatic index: 55.21 </p>
 +
<p> </p>
 +
<p> Grand average of hydropathicity (GRAVY): -0.984 </p>

Latest revision as of 22:01, 18 September 2015

M Chain of CODH/ACS alpha subunit

The alpha subunit of CODH/ACS have four chains, named M,N,O,P. This four chains have same sequence. CODH/ACS has oxidoreductase function， but this function depend on the intact structure,acluding two subunits.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 2210
    Illegal EcoRI site found at 1385
    Illegal EcoRI site found at 2063
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 1385
    Illegal EcoRI site found at 2063
    Illegal SpeI site found at 2211
    Illegal PstI site found at 2225
    Illegal NotI site found at 7
    Illegal NotI site found at 2218
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 1385
    Illegal EcoRI site found at 2063
    Illegal XhoI site found at 1757
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 2211
    Illegal EcoRI site found at 1385
    Illegal EcoRI site found at 2063
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal EcoRI site found at 1385
    Illegal EcoRI site found at 2063
    Illegal XbaI site found at 16
    Illegal SpeI site found at 2211
    Illegal PstI site found at 2225
    Illegal AgeI site found at 73
    Illegal AgeI site found at 1280
  • 1000
    COMPATIBLE WITH RFC[1000]


Parameter of Protein

Number of amino acids: 681

Molecular weight: 79647.4

Theoretical pI: 11.06

Amino acid composition: 

Ala (A) 44 6.5%

Arg (R) 121 17.8%

Asn (N) 21 3.1%

Asp (D) 28 4.1%

Cys (C) 30 4.4%

Gln (Q) 17 2.5%

Glu (E) 12 1.8%

Gly (G) 54 7.9%

His (H) 38 5.6%

Ile (I) 12 1.8%

Leu (L) 59 8.7%

Lys (K) 10 1.5%

Met (M) 1 0.1%

Phe (F) 17 2.5%

Pro (P) 66 9.7%

Ser (S) 55 8.1%

Thr (T) 18 2.6%

Trp (W) 17 2.5%

Tyr (Y) 42 6.2%

Val (V) 19 2.8%

Pyl (O) 0 0.0%

Sec (U) 0 0.0%

(B) 0 0.0%

(Z) 0 0.0%

(X) 0 0.0%

Total number of negatively charged residues (Asp + Glu): 40

Total number of positively charged residues (Arg + Lys): 131

Atomic composition:Carbon C 3496

Hydrogen H 5383

Nitrogen N 1185

Oxygen O 915

Sulfur S 31

Formula: C3496H5383N1185O915S31Total number of atoms: 11010

Extinction coefficients:Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water.

Ext. coefficient 157955

Abs 0.1% (=1 g/l) 1.983, assuming all pairs of Cys residues form cystines

Ext. coefficient 156080

Abs 0.1% (=1 g/l) 1.960, assuming all Cys residues are reduced

Estimated half-life:The N-terminal of the sequence considered is E (Glu).

The estimated half-life is: 1 hours (mammalian reticulocytes, in vitro).

30 min (yeast, in vivo).

>10 hours (Escherichia coli, in vivo).

Instability index:The instability index (II) is computed to be 74.60

This classifies the protein as unstable.

Aliphatic index: 55.21

Grand average of hydropathicity (GRAVY): -0.984