Difference between revisions of "Part:BBa K1796102"

 
 
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__NOTOC__
 
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<partinfo>BBa_K1796102 short</partinfo>
 
<partinfo>BBa_K1796102 short</partinfo>
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<partinfo>BBa_K1796102 parameters</partinfo>
 
<partinfo>BBa_K1796102 parameters</partinfo>
 
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 +
 +
==Parameter of Protein==
 +
<p> Number of amino acids: 631 </p>
 +
<p> </p>
 +
<p> Molecular weight: 71751.2 </p>
 +
<p> </p>
 +
<p> Theoretical pI: 10.03 </p>
 +
<p> </p>
 +
<p> Amino acid composition:  </p>
 +
<p> </p>
 +
<p> Ala (A)  33      5.2% </p>
 +
<p> </p>
 +
<p> Arg (R)  96      15.2% </p>
 +
<p> </p>
 +
<p> Asn (N)  12      1.9% </p>
 +
<p> </p>
 +
<p> Asp (D)  18      2.9% </p>
 +
<p> </p>
 +
<p> Cys (C)  49      7.8% </p>
 +
<p> </p>
 +
<p> Gln (Q)  22      3.5% </p>
 +
<p> </p>
 +
<p> Glu (E)  14      2.2% </p>
 +
<p> </p>
 +
<p> Gly (G)  60      9.5% </p>
 +
<p> </p>
 +
<p> His (H)  28      4.4% </p>
 +
<p> </p>
 +
<p> Ile (I)  8      1.3% </p>
 +
<p> </p>
 +
<p> Leu (L)  48      7.6% </p>
 +
<p> </p>
 +
<p> Lys (K)  7      1.1% </p>
 +
<p> </p>
 +
<p> Met (M)  1      0.2% </p>
 +
<p> </p>
 +
<p> Phe (F)  15      2.4% </p>
 +
<p> </p>
 +
<p> Pro (P)  59      9.4% </p>
 +
<p> </p>
 +
<p> Ser (S)  74      11.7% </p>
 +
<p> </p>
 +
<p> Thr (T)  16      2.5% </p>
 +
<p> </p>
 +
<p> Trp (W)  12      1.9% </p>
 +
<p> </p>
 +
<p> Tyr (Y)  40      6.3% </p>
 +
<p> </p>
 +
<p> Val (V)  19      3.0% </p>
 +
<p> </p>
 +
<p> Pyl (O)  0      0.0% </p>
 +
<p> </p>
 +
<p> Sec (U)  0      0.0% </p>
 +
<p> </p>
 +
<p> (B)  0          0.0% </p>
 +
<p> </p>
 +
<p> (Z)  0          0.0% </p>
 +
<p> </p>
 +
<p> (X)  0          0.0% </p>
 +
<p> </p>
 +
<p> Total number of negatively charged residues (Asp + Glu): 32 </p>
 +
<p> Total number of positively charged residues (Arg + Lys): 103 </p>
 +
<p> </p>
 +
<p> Atomic composition:Carbon      C              3096 </p>
 +
<p> Hydrogen    H        4766 </p>
 +
<p> Nitrogen    N        1028 </p>
 +
<p> Oxygen      O          860 </p>
 +
<p> Sulfur      S          50 </p>
 +
<p> </p>
 +
<p> Formula: C3096H4766N1028O860S50Total number of atoms: 9800 </p>
 +
<p> </p>
 +
<p> Extinction coefficients:Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water. </p>
 +
<p> </p>
 +
<p> Ext. coefficient  128600 </p>
 +
<p> Abs 0.1% (=1 g/l)  1.792, assuming all pairs of Cys residues form cystines </p>
 +
<p> </p>
 +
<p> </p>
 +
<p> Ext. coefficient  125600 </p>
 +
<p> Abs 0.1% (=1 g/l)  1.750, assuming all Cys residues are reduced </p>
 +
<p> </p>
 +
<p> Estimated half-life:The N-terminal of the sequence considered is E (Glu). </p>
 +
<p> </p>
 +
<p> The estimated half-life is: 1 hours (mammalian reticulocytes, in vitro). </p>
 +
<p>                             30 min (yeast, in vivo). </p>
 +
<p>                             >10 hours (Escherichia coli, in vivo). </p>
 +
<p> </p>
 +
<p> </p>
 +
<p> Instability index:The instability index (II) is computed to be 65.39 </p>
 +
<p> This classifies the protein as unstable. </p>
 +
<p> </p>
 +
<p> </p>
 +
<p> </p>
 +
<p> Aliphatic index: 48.57 </p>
 +
<p> </p>
 +
<p> Grand average of hydropathicity (GRAVY): -0.804 </p>

Latest revision as of 21:56, 18 September 2015

A chain of CODH/ACS beta subunit

The beta subunit of CODH/ACS have four chains, named A,B,C,D. This four chains have same sequence. CODH/ACS has oxidoreductase function， but this function depend on the intact structure,acluding two subunits.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 2042
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 2043
    Illegal PstI site found at 2057
    Illegal NotI site found at 7
    Illegal NotI site found at 2050
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 2043
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 2043
    Illegal PstI site found at 2057
    Illegal AgeI site found at 341
    Illegal AgeI site found at 746
    Illegal AgeI site found at 971
    Illegal AgeI site found at 1046
    Illegal AgeI site found at 1511
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1311


Parameter of Protein

Number of amino acids: 631

Molecular weight: 71751.2

Theoretical pI: 10.03

Amino acid composition: 

Ala (A) 33 5.2%

Arg (R) 96 15.2%

Asn (N) 12 1.9%

Asp (D) 18 2.9%

Cys (C) 49 7.8%

Gln (Q) 22 3.5%

Glu (E) 14 2.2%

Gly (G) 60 9.5%

His (H) 28 4.4%

Ile (I) 8 1.3%

Leu (L) 48 7.6%

Lys (K) 7 1.1%

Met (M) 1 0.2%

Phe (F) 15 2.4%

Pro (P) 59 9.4%

Ser (S) 74 11.7%

Thr (T) 16 2.5%

Trp (W) 12 1.9%

Tyr (Y) 40 6.3%

Val (V) 19 3.0%

Pyl (O) 0 0.0%

Sec (U) 0 0.0%

(B) 0 0.0%

(Z) 0 0.0%

(X) 0 0.0%

Total number of negatively charged residues (Asp + Glu): 32

Total number of positively charged residues (Arg + Lys): 103

Atomic composition:Carbon C 3096

Hydrogen H 4766

Nitrogen N 1028

Oxygen O 860

Sulfur S 50

Formula: C3096H4766N1028O860S50Total number of atoms: 9800

Extinction coefficients:Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water.

Ext. coefficient 128600

Abs 0.1% (=1 g/l) 1.792, assuming all pairs of Cys residues form cystines

Ext. coefficient 125600

Abs 0.1% (=1 g/l) 1.750, assuming all Cys residues are reduced

Estimated half-life:The N-terminal of the sequence considered is E (Glu).

The estimated half-life is: 1 hours (mammalian reticulocytes, in vitro).

30 min (yeast, in vivo).

>10 hours (Escherichia coli, in vivo).

Instability index:The instability index (II) is computed to be 65.39

This classifies the protein as unstable.

Aliphatic index: 48.57

Grand average of hydropathicity (GRAVY): -0.804