Difference between revisions of "Part:BBa K1796101"
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<partinfo>BBa_K1796101 short</partinfo> | <partinfo>BBa_K1796101 short</partinfo> | ||
| − | This sequence | + | This sequence codes a HSP, which is helping CODH to fold in right way. If you need to produce CODH and want it to have functions, this protein is necessary. |
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<partinfo>BBa_K1796101 parameters</partinfo> | <partinfo>BBa_K1796101 parameters</partinfo> | ||
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| + | ==Parameter of Protein== | ||
| + | <p> Number of amino acids: 249 </p> | ||
| + | <p> </p> | ||
| + | <p> Molecular weight: 26876.2 </p> | ||
| + | <p> </p> | ||
| + | <p> Theoretical pI: 5.65 </p> | ||
| + | <p> </p> | ||
| + | <p> Amino acid composition: </p> | ||
| + | <p> </p> | ||
| + | <p> Ala (A) 20 8.0% </p> | ||
| + | <p> </p> | ||
| + | <p> Arg (R) 12 4.8% </p> | ||
| + | <p> </p> | ||
| + | <p> Asn (N) 9 3.6% </p> | ||
| + | <p> </p> | ||
| + | <p> Asp (D) 11 4.4% </p> | ||
| + | <p> </p> | ||
| + | <p> Cys (C) 2 0.8% </p> | ||
| + | <p> </p> | ||
| + | <p> Gln (Q) 9 3.6% </p> | ||
| + | <p> </p> | ||
| + | <p> Glu (E) 17 6.8% </p> | ||
| + | <p> </p> | ||
| + | <p> Gly (G) 26 10.4% </p> | ||
| + | <p> </p> | ||
| + | <p> His (H) 4 1.6% </p> | ||
| + | <p> </p> | ||
| + | <p> Ile (I) 25 10.0% </p> | ||
| + | <p> </p> | ||
| + | <p> Leu (L) 29 11.6% </p> | ||
| + | <p> </p> | ||
| + | <p> Lys (K) 12 4.8% </p> | ||
| + | <p> </p> | ||
| + | <p> Met (M) 7 2.8% </p> | ||
| + | <p> </p> | ||
| + | <p> Phe (F) 9 3.6% </p> | ||
| + | <p> </p> | ||
| + | <p> Pro (P) 9 3.6% </p> | ||
| + | <p> </p> | ||
| + | <p> Ser (S) 11 4.4% </p> | ||
| + | <p> </p> | ||
| + | <p> Thr (T) 18 7.2% </p> | ||
| + | <p> </p> | ||
| + | <p> Trp (W) 0 0.0% </p> | ||
| + | <p> </p> | ||
| + | <p> Tyr (Y) 4 1.6% </p> | ||
| + | <p> </p> | ||
| + | <p> Val (V) 15 6.0% </p> | ||
| + | <p> </p> | ||
| + | <p> Pyl (O) 0 0.0% </p> | ||
| + | <p> </p> | ||
| + | <p> Sec (U) 0 0.0% </p> | ||
| + | <p> </p> | ||
| + | <p> (B) 0 0.0% </p> | ||
| + | <p> </p> | ||
| + | <p> (Z) 0 0.0% </p> | ||
| + | <p> </p> | ||
| + | <p> (X) 0 0.0% </p> | ||
| + | <p> </p> | ||
| + | <p> Total number of negatively charged residues (Asp + Glu): 28 </p> | ||
| + | <p> Total number of positively charged residues (Arg + Lys): 24 </p> | ||
| + | <p> </p> | ||
| + | <p> Atomic composition:Carbon C 1197 </p> | ||
| + | <p> Hydrogen H 1959 </p> | ||
| + | <p> Nitrogen N 323 </p> | ||
| + | <p> Oxygen O 357 </p> | ||
| + | <p> Sulfur S 9 </p> | ||
| + | <p> </p> | ||
| + | <p> Formula: C1197H1959N323O357S9Total number of atoms: 3845 </p> | ||
| + | <p> </p> | ||
| + | <p> Extinction coefficients:This protein does not contain any Trp residues. Experience shows that </p> | ||
| + | <p> this could result in more than 10% error in the computed extinction coefficient. </p> | ||
| + | <p> </p> | ||
| + | <p> Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. </p> | ||
| + | <p> </p> | ||
| + | <p> Ext. coefficient 6085 </p> | ||
| + | <p> Abs 0.1% (=1 g/l) 0.226, assuming all pairs of Cys residues form cystines </p> | ||
| + | <p> </p> | ||
| + | <p> </p> | ||
| + | <p> Ext. coefficient 5960 </p> | ||
| + | <p> Abs 0.1% (=1 g/l) 0.222, assuming all Cys residues are reduced </p> | ||
| + | <p> </p> | ||
| + | <p> Estimated half-life:The N-terminal of the sequence considered is V (Val). </p> | ||
| + | <p> </p> | ||
| + | <p> The estimated half-life is: 100 hours (mammalian reticulocytes, in vitro). </p> | ||
| + | <p> >20 hours (yeast, in vivo). </p> | ||
| + | <p> >10 hours (Escherichia coli, in vivo). </p> | ||
| + | <p> </p> | ||
| + | <p> </p> | ||
| + | <p> Instability index:The instability index (II) is computed to be 27.68 </p> | ||
| + | <p> This classifies the protein as stable. </p> | ||
| + | <p> </p> | ||
| + | <p> </p> | ||
| + | <p> </p> | ||
| + | <p> Aliphatic index: 110.08 </p> | ||
| + | <p> </p> | ||
| + | <p> Grand average of hydropathicity (GRAVY): 0.157 </p> | ||
Latest revision as of 21:51, 18 September 2015
CODH chaperone
This sequence codes a HSP, which is helping CODH to fold in right way. If you need to produce CODH and want it to have functions, this protein is necessary.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 598
Illegal SpeI site found at 118 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 598
Illegal SpeI site found at 118 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 598
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 598
Illegal SpeI site found at 118 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 598
Illegal SpeI site found at 118
Illegal NgoMIV site found at 654 - 1000COMPATIBLE WITH RFC[1000]
Parameter of Protein
Number of amino acids: 249
Molecular weight: 26876.2
Theoretical pI: 5.65
Amino acid composition:
Ala (A) 20 8.0%
Arg (R) 12 4.8%
Asn (N) 9 3.6%
Asp (D) 11 4.4%
Cys (C) 2 0.8%
Gln (Q) 9 3.6%
Glu (E) 17 6.8%
Gly (G) 26 10.4%
His (H) 4 1.6%
Ile (I) 25 10.0%
Leu (L) 29 11.6%
Lys (K) 12 4.8%
Met (M) 7 2.8%
Phe (F) 9 3.6%
Pro (P) 9 3.6%
Ser (S) 11 4.4%
Thr (T) 18 7.2%
Trp (W) 0 0.0%
Tyr (Y) 4 1.6%
Val (V) 15 6.0%
Pyl (O) 0 0.0%
Sec (U) 0 0.0%
(B) 0 0.0%
(Z) 0 0.0%
(X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 28
Total number of positively charged residues (Arg + Lys): 24
Atomic composition:Carbon C 1197
Hydrogen H 1959
Nitrogen N 323
Oxygen O 357
Sulfur S 9
Formula: C1197H1959N323O357S9Total number of atoms: 3845
Extinction coefficients:This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.
Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water.
Ext. coefficient 6085
Abs 0.1% (=1 g/l) 0.226, assuming all pairs of Cys residues form cystines
Ext. coefficient 5960
Abs 0.1% (=1 g/l) 0.222, assuming all Cys residues are reduced
Estimated half-life:The N-terminal of the sequence considered is V (Val).
The estimated half-life is: 100 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:The instability index (II) is computed to be 27.68
This classifies the protein as stable.
Aliphatic index: 110.08
Grand average of hydropathicity (GRAVY): 0.157