Difference between revisions of "Part:BBa K1648045:Design"
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===Design Notes===
 
===Design Notes===
−
PCR with BBa_K1314016 as template using the following primers:
+
PCR with Azotobacter vinelandii as template using the following primers:
  
−
>SH3 Domain F<br>
+
Forward primer:
−
GTTTCTTCGAATTCGCGGCCGCTTCTAGatgaaactgaaaaacaccttgggcg
+
GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAAACTGAAAAACACCTTGGGCG
 +
 
 +
Reverse primer:
 +
GTTTCTTCCTGCAGCGGCCGCTACTAGTATTACTGGGCTTGGGCTTCCAC
 +
 
 +
Insert oprF PCR product into psB1C3
  
−
>SH3 Domain R<br>
 
−
GTTTCTTCCTGCAGCGGCCGCTACTAGTAttactgggcttgggcttccac
 
 
===Source===
 
===Source===
−
BBa_K1314016
+
The oprF gene was obtained by PCR from the Azotobacter vinelandii strain DJ genome.
  
 
===References===
 
===References===

Latest revision as of 08:25, 17 September 2015

AvOprF


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 475


Design Notes

PCR with Azotobacter vinelandii as template using the following primers:

Forward primer: GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAAACTGAAAAACACCTTGGGCG

Reverse primer: GTTTCTTCCTGCAGCGGCCGCTACTAGTATTACTGGGCTTGGGCTTCCAC

Insert oprF PCR product into psB1C3

Source

The oprF gene was obtained by PCR from the Azotobacter vinelandii strain DJ genome.

References