Difference between revisions of "Part:BBa K1728025"

 
 
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<partinfo>BBa_K1728025 short</partinfo>
 
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rhodopsin signalling peptide+Chemokine receptor 1 (CXCR1)
 
rhodopsin signalling peptide+Chemokine receptor 1 (CXCR1)
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===Usage and Biology===
  
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==Construction of new yeast integrating plasmids==
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===Usage and Biology===
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Plasmids cloning vectors that can “shuttle” DNA between yeast and bacteria we used is called pGAL426. Distinctly, it has different selection markers in yeast and bacteria. Lucy-rho-CXCR1 was inserted into this shuttle vector and finally transformed into FAR1△::KanMX-FUS1-GFP Gpa1 chimera strain.
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https://static.igem.org/mediawiki/2015/3/3a/CGU-pGal426_Lucy-rho-CXCR1_with_BamHI_and_XhoI_digestion.jpg
  
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The result of pGal426 Lucy-rho-CXCR1 with BamHI and XhoI digestion.
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Note: These two restriction enzyme site designed on insert sequence was used to check the ligation performance by enzyme digestion. Theoretically, there would be two bands.
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Latest revision as of 16:05, 19 November 2015

LUCY rhodopsin signalling peptide+Chemokine receptor 1 (CXCR1)

rhodopsin signalling peptide+Chemokine receptor 1 (CXCR1)

Usage and Biology

Construction of new yeast integrating plasmids

Plasmids cloning vectors that can “shuttle” DNA between yeast and bacteria we used is called pGAL426. Distinctly, it has different selection markers in yeast and bacteria. Lucy-rho-CXCR1 was inserted into this shuttle vector and finally transformed into FAR1△::KanMX-FUS1-GFP Gpa1 chimera strain. CGU-pGal426_Lucy-rho-CXCR1_with_BamHI_and_XhoI_digestion.jpg

The result of pGal426 Lucy-rho-CXCR1 with BamHI and XhoI digestion. Note: These two restriction enzyme site designed on insert sequence was used to check the ligation performance by enzyme digestion. Theoretically, there would be two bands. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 909
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 334
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 49
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1098
    Illegal BsaI.rc site found at 63