Difference between revisions of "Part:BBa K1602029"
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+ | This part is capable of generating the whole protein scaffold containing the GBD,PDZ and SH3 domains under the control of a T7 promotor. This scaffold was used by the iGEM Team TU Darmstadt 2015 to introduce a <i>in vitro</i> immobilized enzyme system. The sequence coding for a His-tag is fused N-terminus, improving the purification yield. Also the production is codon optimized for growth in <i>E. coli</i> using part <a href=" https://parts.igem.org/Part:BBa_B0034">BBa_B0034</a>. | ||
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Latest revision as of 01:23, 19 September 2015
Inducible generator of protein scaffold with N-terminal HisTag
This part is capable of generating the whole protein scaffold containing the GBD,PDZ and SH3 domains under the control of a T7 promotor. This scaffold was used by the iGEM Team TU Darmstadt 2015 to introduce a in vitro immobilized enzyme system. The sequence coding for a His-tag is fused N-terminus, improving the purification yield. Also the production is codon optimized for growth in E. coli using part BBa_B0034. |
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 71
Illegal BamHI site found at 926 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 143
Illegal AgeI site found at 266 - 1000COMPATIBLE WITH RFC[1000]