Difference between revisions of "Part:BBa K1678002"

(Created page with "Bacillus subtilis RibT gene for riboflavin production. RibT code for the Beta-chain of the riboflavin synthase. Coding sequence has been codon optimized for Lactobacillus planta...")
 
 
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Bacillus subtilis RibT gene for riboflavin production. RibT code for the Beta-chain of the riboflavin synthase.  
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__NOTOC__
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Coding sequence has been codon optimized for Lactobacillus plantarum.  
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<partinfo>BBa_K1678002 short</partinfo>
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Protein is expressed by a L.plantarum synthetic promoter p25 (A synthetic promoter library for constitutive gene expression in Lactobacillus plantarum, Microbiology (2006), 152, 1011–1019).  
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The gene is terminated by the Tldh terminator (Spath et al. Microbial Cell Factories 2012, 11:141).
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Bacillus subtilis RibT gene for riboflavin production. RibT code for the Beta-chain of the riboflavin synthase. <br>
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The RBS and the spacing between the RBS and the coding sequence are optimized for Lactobacillus plantarum (Tauer et al. Microbial Cell Factories 2014, 13:150).  
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Coding sequence has been codon optimized for Lactobacillus plantarum. <br>
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The expressed protein catalyses two steps in the riboflavin pathway.
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Protein is expressed by a L.plantarum synthetic promoter p25 (A synthetic promoter library for constitutive gene expression in Lactobacillus plantarum, Microbiology (2006), 152, 1011–1019). <br>
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The gene is terminated by the Tldh terminator (Spath et al. Microbial Cell Factories 2012, 11:141).<br>
 +
The RBS and the spacing between the RBS and the coding sequence are optimized for Lactobacillus plantarum (Tauer et al. Microbial Cell Factories 2014, 13:150). <br>
 +
The expressed protein catalyses two steps in the riboflavin pathway.<br>
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<!-- Add more about the biology of this part here
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===Usage and Biology===
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<!-- -->
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K1678002 SequenceAndFeatures</partinfo>
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<!-- Uncomment this to enable Functional Parameter display
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===Functional Parameters===
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<partinfo>BBa_K1678002 parameters</partinfo>
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<!-- -->

Latest revision as of 11:55, 21 September 2015

RibT promoted by p25 synthetic promoter

Bacillus subtilis RibT gene for riboflavin production. RibT code for the Beta-chain of the riboflavin synthase.
Coding sequence has been codon optimized for Lactobacillus plantarum.
Protein is expressed by a L.plantarum synthetic promoter p25 (A synthetic promoter library for constitutive gene expression in Lactobacillus plantarum, Microbiology (2006), 152, 1011–1019).
The gene is terminated by the Tldh terminator (Spath et al. Microbial Cell Factories 2012, 11:141).
The RBS and the spacing between the RBS and the coding sequence are optimized for Lactobacillus plantarum (Tauer et al. Microbial Cell Factories 2014, 13:150).
The expressed protein catalyses two steps in the riboflavin pathway.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 22
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 553