Difference between revisions of "Part:BBa K1820021"

 
 
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<partinfo>BBa_K1820021 short</partinfo>
 
<partinfo>BBa_K1820021 short</partinfo>
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<p>A phage-inducible promoter natively located in the middle of the genome of the P335 species lactococcal phage Φ31 with the B0034 ribosomal binding site (RBS) (BBa_K1820001), a red fluorescent protein from the 2013 Uppsala team, previously termed mCherry, that was codon optimized for ''Lactobacillus reuteri'' (BBa_K1033250), and a transcriptional terminator.</p>
  
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===Usage and Biology===
 
===Usage and Biology===
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<p> This construct was designed to initiate transcription after phage infection of ''Lactococcus lactis'', resulting in expression of mCherry (previously shown to have fluorescent activity in ''L. lactis''). Designed to allow for fluorescent detection of Φ31 phage infection in ''L. lactis''.</p>
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<p> This construct has yet to be cloned and expressed in ''L. lactis'', and validated for functionality.</p>
  
 
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<span class='h3bb'>Sequence and Features</span>
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===Sequence and Features===
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<partinfo>BBa_K1820021 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1820021 SequenceAndFeatures</partinfo>
  

Latest revision as of 16:11, 26 September 2015

P335-Φ31 inducible middle promoter + RBS + mCherry + term

A phage-inducible promoter natively located in the middle of the genome of the P335 species lactococcal phage Φ31 with the B0034 ribosomal binding site (RBS) (BBa_K1820001), a red fluorescent protein from the 2013 Uppsala team, previously termed mCherry, that was codon optimized for Lactobacillus reuteri (BBa_K1033250), and a transcriptional terminator.

Usage and Biology

This construct was designed to initiate transcription after phage infection of Lactococcus lactis, resulting in expression of mCherry (previously shown to have fluorescent activity in L. lactis). Designed to allow for fluorescent detection of Φ31 phage infection in L. lactis.

This construct has yet to be cloned and expressed in L. lactis, and validated for functionality.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 42