Difference between revisions of "Part:BBa K1820020"

 
 
(5 intermediate revisions by 2 users not shown)
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1820020 short</partinfo>
 
<partinfo>BBa_K1820020 short</partinfo>
 +
<p>A phage-inducible promoter natively located in the middle of the genome of the P335 species lactococcal phage Φ31 with the B0034 ribosomal binding site (RBS) (BBa_K1820001), a super-folded green fluorescent protein (sfGFP) from the 2014 Groningen team that was codon optimized for ''Bacillus subtilis'' (BBa_K1365020), and a transcriptional terminator.</p>
  
−
Add
 
−
 
−
<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
  
 +
<p> This construct was designed to initiate transcription after phage infection of ''Lactococcus lactis'', resulting in expression of sfGFP (previously shown to have a high level of fluorescent activity in ''L. lactis''). Allowing for fluorescent detection of Φ31 phage infection in ''L. lactis''.</p>
 +
 +
<p> This construct has yet to be cloned and expressed in ''L. lactis'', and validated for functionality.</p>
 
<!-- -->
 
<!-- -->
−
<span class='h3bb'>Sequence and Features</span>
+
 
 +
===Sequence and Features===
 +
<span class='h3bb'></span>
 
<partinfo>BBa_K1820020 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1820020 SequenceAndFeatures</partinfo>
  

Latest revision as of 16:10, 26 September 2015

P335-Φ31 inducible middle promoter + RBS+ sfGFP + term

A phage-inducible promoter natively located in the middle of the genome of the P335 species lactococcal phage Φ31 with the B0034 ribosomal binding site (RBS) (BBa_K1820001), a super-folded green fluorescent protein (sfGFP) from the 2014 Groningen team that was codon optimized for Bacillus subtilis (BBa_K1365020), and a transcriptional terminator.

Usage and Biology

This construct was designed to initiate transcription after phage infection of Lactococcus lactis, resulting in expression of sfGFP (previously shown to have a high level of fluorescent activity in L. lactis). Allowing for fluorescent detection of Φ31 phage infection in L. lactis.

This construct has yet to be cloned and expressed in L. lactis, and validated for functionality.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 42
    Illegal SapI.rc site found at 284