Difference between revisions of "Part:BBa K1679020"

Latest revision as of 03:31, 19 September 2015

RBS+ftnA

This is a translational unit containing a RBS (BBa_B0034) and a ftnA on the pSB1C3.

Ferritin

FtnA is a bacterial ferritin with a protein shell is assembled from 24 identical 19.4 kDa FtnA monomers. Its central cavity is around 7.5 nm in diameter and can be loaded with iron when cells grow under iron-rich conditions[1]. The iron is stored in the form of ferrihydrite iron cores normally that with superparamagnetic properties[2]. The iron contained ferritin can generate heat in response to electromagnetic signal[3]. For the reasons above, we design it as our magnetic receiver which can turn electromagnetic signal into heat.

[1]Smith J L. The physiological role of ferritin-like compounds in bacteria[J]. Critical reviews in microbiology, 2004, 30(3): 173-185. [2] Papaefthymiou G C, Viescas A J, Devlin E, et al. Electronic and magnetic characterization of in vivo produced vs. in vitro reconstituted horse spleen ferritin[C]//MRS Proceedings. Cambridge University Press, 2007, 1056: 1056-HH03-27. [3] Stanley S A, Sauer J, Kane R S, et al. Remote regulation of glucose homeostasis in mice using genetically encoded nanoparticles[J]. Nature medicine, 2015, 21(1): 92-98.

Fig.1. Schema of ferritin

Experiment

We purified the ferritin overexpressed in E.coli through Ni-chelating affinity chromatography and highly concentrated it and then do SDS-PAGE. The position of clearly targeted band(about 19.4kDA) on the gel was consistent with the size of ferritin monomer fused with tag on plasmid, explaining that the ftnA expression is successful.

Figure 2. SDS-PAGE shows the expected protein band.

Gel was stained with (A) potassium ferrocyanide and (B) Coomassie Brilliant Blue R250. Control, HFn; lane 1, concentrated mineralized ferritin purified product; lane 2, sediment of bacteria with mineralization; lane 3, concentrated unmineralized ferritin purified product ; lane 4, sediment of bacteria without mineralization.

Figure 3. Native-PAGE analysis of mineralized FtnA

Figure 4.(a) Low‐Temperature Magnetization Curves(5K) (b) Enlarge figure of the left figure, Low‐Field Magnetization Curves

Figure5.(a) Magnetization Curves at 300K (b) Enlarge figure of the left figure

Figure6.Decay curves of saturation isothermal remanent magnetization(IRM) acquired in a 2.5 T field after zero‐field cooling (ZFC) and field‐cooling (FC) treatments Normalized IRM acquisition and DC (Direct Current field) demagnetization (DCD), measured at 5 K

The picture show the magnetism properties of iron core of Ftna Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1679020 short</partinfo>
-This is a translational unit containing a RBS (BBa_B0034) and a ftnA on the pSB1C3.
+This is a translational unit containing a RBS (BBa_B0034) and a [https://parts.igem.org/Part:BBa_K1679029 ftnA] on the pSB1C3.
+===Ferritin===
+FtnA is a bacterial ferritin with a protein shell is assembled from 24 identical 19.4 kDa FtnA monomers. Its central cavity is around 7.5 nm in diameter and can be loaded with iron when cells grow under iron-rich
+conditions[1]. The iron is stored in the form of ferrihydrite iron cores normally that with superparamagnetic properties[2]. The iron contained ferritin can generate heat in response to electromagnetic signal[3]. For the reasons above, we design it as our magnetic receiver which can turn electromagnetic signal into heat.
+[1]Smith J L. The physiological role of ferritin-like compounds in bacteria[J]. Critical reviews in microbiology, 2004, 30(3): 173-185.
+[2] Papaefthymiou G C, Viescas A J, Devlin E, et al. Electronic and magnetic characterization of in vivo produced vs. in vitro reconstituted horse spleen ferritin[C]//MRS Proceedings. Cambridge University Press, 2007, 1056: 1056-HH03-27.
+[3] Stanley S A, Sauer J, Kane R S, et al. Remote regulation of glucose homeostasis in mice using genetically encoded nanoparticles[J]. Nature medicine, 2015, 21(1): 92-98.
+[[File:ftnA-ouc.jpg|250px|thumb|centre|Fig.1. Schema of ferritin]]
-<!-- Add more about the biology of this part here
+===Experiment===
-===Usage and Biology===
+We purified the ferritin overexpressed in E.coli through Ni-chelating affinity chromatography and highly concentrated it and then do SDS-PAGE. The position of clearly targeted band(about 19.4kDA) on the gel was consistent with the size of ferritin monomer fused with tag on plasmid, explaining that the ftnA expression is successful.
+[[File:ferritin concentrated purified product SDS-PAGE.jpg|500px|thumb|centre|Figure 2. SDS-PAGE shows the expected protein band.]]
+Gel was stained with (A) potassium ferrocyanide and (B) Coomassie Brilliant Blue R250. Control, HFn;
+lane 1, concentrated mineralized ferritin purified product; lane 2, sediment of bacteria with mineralization; lane 3, concentrated unmineralized ferritin purified product ; lane 4, sediment of bacteria without mineralization.
+[[File:ftnA_native page.jpg|500px|thumb|centre|Figure 3. Native-PAGE analysis of mineralized FtnA
+]]
+[[File:zxh199501101.png|500px|thumb|centre|Figure 4.(a)	Low‐Temperature Magnetization Curves(5K) (b)	Enlarge figure of the left figure, Low‐Field Magnetization Curves ]]
+[[File:zxh199501102.png|500px|thumb|centre|Figure5.(a) Magnetization Curves at 300K (b) Enlarge figure of the left figure]]
+[[File:zxh199501103.png|500px|thumb|centre|Figure6.Decay curves of saturation isothermal remanent magnetization(IRM) acquired in a 2.5 T field after zero‐field cooling (ZFC) and field‐cooling (FC) treatments Normalized IRM acquisition and DC (Direct Current field) demagnetization (DCD), measured at 5 K ]]
+The picture show the magnetism properties of iron core of Ftna
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 <span class='h3bb'>Sequence and Features</span>