Difference between revisions of "Part:BBa K1628004"

 
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<partinfo>BBa_K1628004 short</partinfo>
 
<partinfo>BBa_K1628004 short</partinfo>
  
Promoter C2up is an artificially synthesized promoter. According to our promoter strength assay in ''B. amyloliquefaciens'' NK-1 (showed in Figure 1), C2up is the strongest promoters we used in our project.
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Promoter C2up is an artificially synthesized promoter. We have validate this part through promoter strength assay using ''bgaB'' (encoding β-galatosidase downstream of the multiple cloning sites) as reporter gene. According to promoter strength assay in ''B. amyloliquefaciens'' NK-1 (showed in Figure 1), C2up is the strongest promoters we used in our project.
  
[[File:promoter2_NK.png]]
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[[File:promoter4_NK.png]]
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 09:04, 18 September 2015

C2up

Promoter C2up is an artificially synthesized promoter. We have validate this part through promoter strength assay using bgaB (encoding β-galatosidase downstream of the multiple cloning sites) as reporter gene. According to promoter strength assay in B. amyloliquefaciens NK-1 (showed in Figure 1), C2up is the strongest promoters we used in our project.

Promoter4 NK.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]