Difference between revisions of "Part:BBa K1679004"

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BBa_J23101 is a strong promoter which is well characterized by many teams who take part in 2015 InterLab Study. RiboJ is a Ribozyme-based insulator part that can buffer synthetic circuits. After transcription, RiboJ becomes a section of functional RNA which comprises the sTRSV- ribozyme with an additional 23-nt hairpin immediately downstream to help expose the RBS. This part can be used as a promoter-like part.
 
BBa_J23101 is a strong promoter which is well characterized by many teams who take part in 2015 InterLab Study. RiboJ is a Ribozyme-based insulator part that can buffer synthetic circuits. After transcription, RiboJ becomes a section of functional RNA which comprises the sTRSV- ribozyme with an additional 23-nt hairpin immediately downstream to help expose the RBS. This part can be used as a promoter-like part.
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<h3>Attention Please!</h3>
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The right sequence should be "tttacagctagctcagtcctaggtattatgctagctactagagagctgtcaccggatgtgctttccggtctgatgagtccgtgaggacgaaacagcctctacaaataattttgtttaa".
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But the DNA Sample we submit is correct.
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We are so sorry for this problem.
  
 
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<!-- Add more about the biology of this part here

Latest revision as of 10:35, 10 October 2016

J23101 and RiboJ

BBa_J23101 is a strong promoter which is well characterized by many teams who take part in 2015 InterLab Study. RiboJ is a Ribozyme-based insulator part that can buffer synthetic circuits. After transcription, RiboJ becomes a section of functional RNA which comprises the sTRSV- ribozyme with an additional 23-nt hairpin immediately downstream to help expose the RBS. This part can be used as a promoter-like part.

Attention Please!

The right sequence should be "tttacagctagctcagtcctaggtattatgctagctactagagagctgtcaccggatgtgctttccggtctgatgagtccgtgaggacgaaacagcctctacaaataattttgtttaa". But the DNA Sample we submit is correct. We are so sorry for this problem.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]