Difference between revisions of "Part:BBa K1614010"
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<partinfo>BBa_K1614010 short</partinfo> | <partinfo>BBa_K1614010 short</partinfo> | ||
{{Template:ssDNA}}<br/> | {{Template:ssDNA}}<br/> | ||
| − | His Tag II AptaBody is a sequence consisting of a His-Tag aptamer and the HRP-mimicking DNAzyme for use in Western Blot applications. Between the His Tag aptamer and the HRP mimicking DNAzyme a 5x poly-A spacer is placed ensuring the functionality for ligand binding and peroxidase activity. | + | His Tag II <b>AptaBody</b> is a sequence consisting of a His-Tag aptamer and the HRP-mimicking DNAzyme for use in Western Blot applications. Between the His Tag aptamer and the HRP mimicking DNAzyme a 5x poly-A spacer is placed ensuring the functionality for ligand binding and peroxidase activity. |
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| + | For optimal hydrogen peroxide degradation, hemin should be contained in the solution in equimolar concentration to the <b>AptaBody</b> and pH should be 8.5. | ||
Latest revision as of 19:51, 20 September 2015
His Tag II AptaBody (A5)
Notice: Functional DNA
This part is a sequence of a functional ssDNA. It is only active as single-stranded DNA. It can not be cloned into a plasmid. For use order it as a DNA oligo.
His Tag II AptaBody is a sequence consisting of a His-Tag aptamer and the HRP-mimicking DNAzyme for use in Western Blot applications. Between the His Tag aptamer and the HRP mimicking DNAzyme a 5x poly-A spacer is placed ensuring the functionality for ligand binding and peroxidase activity.
For optimal hydrogen peroxide degradation, hemin should be contained in the solution in equimolar concentration to the AptaBody and pH should be 8.5.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]