Difference between revisions of "Part:BBa K1709003:Design"
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===Design Notes=== | ===Design Notes=== | ||
1 bp in BBa_K629003 was adapted to create an original restriciton site in the plasmid. CheZ was fused to GFP (BBa_K082003) and its LVA tag was codon-optimized to avoid introducing artificial DNA repetitions. | 1 bp in BBa_K629003 was adapted to create an original restriciton site in the plasmid. CheZ was fused to GFP (BBa_K082003) and its LVA tag was codon-optimized to avoid introducing artificial DNA repetitions. | ||
| − | + | gBlocks were used to partly design the plasmid and the ribosome binding site sequence was based purely on the Anderson library and thus, there is a mistake in the composite part sequence since the scar is only partly present. | |
| − | + | ||
===Source=== | ===Source=== | ||
Latest revision as of 17:26, 18 September 2015
CheZ-GFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1307
Design Notes
1 bp in BBa_K629003 was adapted to create an original restriciton site in the plasmid. CheZ was fused to GFP (BBa_K082003) and its LVA tag was codon-optimized to avoid introducing artificial DNA repetitions. gBlocks were used to partly design the plasmid and the ribosome binding site sequence was based purely on the Anderson library and thus, there is a mistake in the composite part sequence since the scar is only partly present.
Source
DNA sequences originate from the E. Coli genome and previous biobrick sequences. The ribosome binding site was chosen from the Anderson RBS family.