Difference between revisions of "Part:BBa K653000:Experience"
 
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===Applications of BBa_K653000===
 
===Applications of BBa_K653000===
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<h4>Experience of the ITB_Indonesia iGEM 2015 team.</h4>
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The iGEM 2015 ITB Indonesia used this coding sequence to make BBa_K1685011 part. The Biosurfactor was placed under T7lac promoter and RBS (BBa_K613010) with a double terminator (BBa_B0015) for rhamnolipid production in <i>E. coli</i> BL21(DE3). After IPTG induction, the separated supernatant was tested for surfactant activity. <br>
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Supernatant from empty cell, either induced or not, had no / little surfactant activity. The uninduced transformant had little activity, but the induced transformant had higher surfactant activity.<br>
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This shows that the part was induced for enzyme expression and biosurfactant production. To our knowledge, this is the very first time that the part was used for expression.
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===User Reviews===
 
===User Reviews===
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<I>ITB_Indonesia 2015</I>
 
<I>ITB_Indonesia 2015</I>
 
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ITB_Indonesia expressed this part under T7lac and strong RBS (BBa_K613010) and terminated by double terminator (BBa_B0015) in E. coli BL21 (DE3). The supernatant showed surfactant activity.
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Latest revision as of 03:37, 19 September 2015

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K653000

Experience of the ITB_Indonesia iGEM 2015 team.

The iGEM 2015 ITB Indonesia used this coding sequence to make BBa_K1685011 part. The Biosurfactor was placed under T7lac promoter and RBS (BBa_K613010) with a double terminator (BBa_B0015) for rhamnolipid production in E. coli BL21(DE3). After IPTG induction, the separated supernatant was tested for surfactant activity.
Supernatant from empty cell, either induced or not, had no / little surfactant activity. The uninduced transformant had little activity, but the induced transformant had higher surfactant activity.
This shows that the part was induced for enzyme expression and biosurfactant production. To our knowledge, this is the very first time that the part was used for expression.

User Reviews

UNIQd40c405aa8a41887-partinfo-00000001-QINU UNIQd40c405aa8a41887-partinfo-00000002-QINU