Difference between revisions of "Part:BBa K1859001"
 
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<p>The 3´side of the intron([https://parts.igem.org/Part:BBa_K1332005 BBa_K1332005]) +GGSGGS linker</p>
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<p>The 3´side of the intron
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<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1332005" > [BBa_K1332005]
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</a>  +GGSGGS linker</p>
  
 
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<p>BBa_K1859001 is Circular Parts conjuncted linker to keep folding of proteins.Circular Parts is what use sequence subcloned ribozyme involved self‐splicing in T4 Phage.By associating Circular Parts, the 3'side of intron[BBa_K1332005] and the 5'side of intron[BBa_K1332003], on both ends of optional sequence, you can circularize mRNA making its DNA. </p>
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<p>[BBa_K1859001] is the 3'side of the intron
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<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1332005" > [BBa_K1332005] </a>
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conjuncted linker to keep folding of proteins.</p>
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<p>
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Circular Parts, the 3'side of the intron  
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<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1332005" > [BBa_K1332005] </a>
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and the 5'side of the intron
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<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1332003" > [BBa_K1332003] </a>
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, is what use sequence subcloned ribozyme involved self‐splicing in T4 Phage.
 +
 
 +
By associating Circular Parts on both ends of optional sequence, you can circularize mRNA making its DNA. </p>
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<p>As for details, refer to  
 
<p>As for details, refer to  
 
<a href= "http://2014.igem.org/Team:Gifu" > iGEM Gifu 2014 </a>
 
<a href= "http://2014.igem.org/Team:Gifu" > iGEM Gifu 2014 </a>
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<p>In the study of iGEM Gifu 2014, we made long-chain protein from cyclic mRNA, but it lost functionality.It is thought that folding of the protein failed for a cause．Therefore we designed some linkers to fix the folding of the protein.These parts include sequence of Circular Parts and linker．The linker comes to be in the cyclic mRNA when mRNA is circularized.To make this device, we coupled the sequence of the linker, GGSGGS , to the down stream of 3'intron.</p>
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 +
<p>In the study of iGEM Gifu 2014, we made long-chain protein from Circular mRNA, but it lost functionality.
 +
 
 +
It is thought that folding of the protein failed for a cause．
 +
 
 +
Therefore we designed some linkers to fix the folding of the protein.
 +
 
 +
[BBa_K1859001] includes sequence of the 3'side of the intron
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<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1332005" > [BBa_K1332005] </a>
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and linker．
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The linker comes inside the Circular mRNA when mRNA is circularized.
 +
 
 +
To make this device, we combined the sequence of the linker, GGSGGS , to the downstream of 3'side of the intron
 +
<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1332005" > [BBa_K1332005] </a>
 +
.</p>
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<p>The linker designed elsewhere is  
 
<p>The linker designed elsewhere is  
 
<a href= "http://2015.igem.org/Team:Gifu/Result/" > here </a>.</p>
 
<a href= "http://2015.igem.org/Team:Gifu/Result/" > here </a>.</p>
  
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<br>
  
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<p>
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We attached this part[BBa_K1859001] to 5 'sides of
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His-RFP without stop codon
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<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1332002" > [BBa_K1332002]
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</a> .
  
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Then, 
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it was connected to each 5 'sides of
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<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1859004" > [BBa_K1859004]
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</a>
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and
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<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1859006" > [BBa_K1859006]
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</a>.
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In addition,Lacl
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<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_R0010" >[BBa_R0010] </a>
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, RBS
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<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_B0034" >[BBa_B0034] </a>
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and DT
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<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_B0015" >[BBa_B0015] </a>
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was conbined with them, making
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<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1859027" >[BBa_K1859027] </a>
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and
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<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1859025" >[BBa_K1859025] </a>
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.
 +
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<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1859027" >[BBa_K1859027] </a>
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is the generator that express long-chain protein with keeping functionality of the protein.
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<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1859025" >[BBa_K1859025] </a>
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is the generator to improve circular efficiency of mRNA including His-RFP.
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 +
</p>
  
 
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Latest revision as of 09:46, 22 September 2015

The 3´side of the intron [BBa_K1332005] +GGSGGS linker


[BBa_K1859001] is the 3'side of the intron [BBa_K1332005] conjuncted linker to keep folding of proteins.

Circular Parts, the 3'side of the intron [BBa_K1332005] and the 5'side of the intron [BBa_K1332003] , is what use sequence subcloned ribozyme involved self‐splicing in T4 Phage. By associating Circular Parts on both ends of optional sequence, you can circularize mRNA making its DNA.

As for details, refer to iGEM Gifu 2014


In the study of iGEM Gifu 2014, we made long-chain protein from Circular mRNA, but it lost functionality. It is thought that folding of the protein failed for a cause． Therefore we designed some linkers to fix the folding of the protein. [BBa_K1859001] includes sequence of the 3'side of the intron [BBa_K1332005] and linker． The linker comes inside the Circular mRNA when mRNA is circularized. To make this device, we combined the sequence of the linker, GGSGGS , to the downstream of 3'side of the intron [BBa_K1332005] .

The linker designed elsewhere is here .


We attached this part[BBa_K1859001] to 5 'sides of His-RFP without stop codon [BBa_K1332002] . Then, it was connected to each 5 'sides of [BBa_K1859004] and [BBa_K1859006] . In addition,Lacl [BBa_R0010] , RBS [BBa_B0034] and DT [BBa_B0015] was conbined with them, making [BBa_K1859027] and [BBa_K1859025] . [BBa_K1859027] is the generator that express long-chain protein with keeping functionality of the protein. [BBa_K1859025] is the generator to improve circular efficiency of mRNA including His-RFP.