Difference between revisions of "Part:BBa K1230000"

Latest revision as of 22:27, 19 October 2016

AcrAB-TolC multidrug efflux transport system activator from E. coli

The MarA protein acts as a transcriptional activator in E. coli, it allows the expression of the acrAB and tolC operons, which activate the AcrAB-TolC efflux pump, a mechanism that has been related with resistance to organic solvents, dyes, detergents, antibiotics such as chloramphenicol, tetracycline, novobiocin, erythromycin, fusidic acid and cloxacillin, as well as to cationic antimicrobial peptides, such as LL-37, HNP-2 and HBD-1.

We used device BBa_K1230004 to characterise this part.

Team iGEM Nagahama 2015
We improved the characterization of a previously existing BioBrick Part BBa_K1230000 and submitted this improved BioBrick marA gene as BBa_K1653006 to iGEM Registry.

Team Nagahama 2016
We improved the characterization of existing this Part BBa_K1230000. We found that marA expression is also improve resistance to farnesol. Please see our wiki result page [http://2016.igem.org/Team:Nagahama/Result_and_Discussion ""Enhancement of farnesol resistance"".]

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 154
1000
COMPATIBLE WITH RFC[1000]

@@ Line 8: / Line 8: @@
 Team iGEM Nagahama 2015
 <br>
-We improved the characterization of a previously existing BioBrick Part [https://parts.igem.org/Part:BBa_K1230000 BBa_K1230000] and submitted this improved BioBrick ''marA'' device as [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1653006 BBa_K1653006] to iGEM Registry.
+We improved the characterization of a previously existing BioBrick Part [https://parts.igem.org/Part:BBa_K1230000 BBa_K1230000] and submitted this improved BioBrick ''marA'' gene as [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1653006 BBa_K1653006] to iGEM Registry.
+Team Nagahama 2016
+<br>
+We improved the characterization of existing this Part [https://parts.igem.org/Part:BBa_K1230000 BBa_K1230000]. We found that marA expression is also improve resistance to farnesol. Please see our wiki result page [http://2016.igem.org/Team:Nagahama/Result_and_Discussion ""Enhancement of farnesol resistance"".]