Difference between revisions of "Part:BBa K1631000"
 
(9 intermediate revisions by the same user not shown)
Line 2: Line 2:
 
<partinfo>BBa_K1631000 short</partinfo>
 
<partinfo>BBa_K1631000 short</partinfo>
  
−
<p>This part is improvement of <a href="https://parts.igem.org/Part:BBa_I716211">BBa_I716211</a>. We added a start codon and charactarized it. If you want to know more detail, visit our wiki.
+
<p>This part is improvement of [[Part:BBa_I716211]]. We added a start codon and charactarized it. If you want to know more detail, visit our wiki.
−
<br>Barnase is a RNase from <i>Bacillus amyloliquefaciens</i>. Its cytotoxity can be neutralized by immunity protein called Barstar (BBa_K1631007).
+
<br>Barnase is a RNase from <i>Bacillus amyloliquefaciens</i>. Its cytotoxity can be neutralized by immunity protein called Barstar ([[Part:BBa_K1631007]]).
 
<br>This coding sequence only contains catalitic domain (not include extracellular translocation signal peptide of <i>Bacillus amyloliquefaciens</i>).</P>
 
<br>This coding sequence only contains catalitic domain (not include extracellular translocation signal peptide of <i>Bacillus amyloliquefaciens</i>).</P>
  
  
−
<!-- Add more about the biology of this part here
+
 
 +
<!-- <a href="https://parts.igem.org/Part:BBa_I716211">BBa_I716211</a>
 +
 
 +
 
 +
<!-- Add more about the biology of this part here -->
 
===Usage and Biology===
 
===Usage and Biology===
 +
We succeeded to control growth rate of <i>E coli</i>.
 +
<p>We induced this parts and [Plac -rbs- GFP -d.term] with 100μM IPTG. [Plac -rbs- GFP -d.term] is negative control.
 +
We measured OD600 of 3 samples for each condition.
 +
[[File:Yamanaka 002.png|600px|thumb|center|alt text]]
 +
</p>
 +
 +
We also evaluated the growth rate by slope of next graph.
 +
[[File:Yamanaka 001.png|600px|thumb|center|alt text]]
 +
<br>Plac GFP-          0.0213(/min)
 +
<br>Plac GFP+          0.0205(/min)
 +
<br>Plac Barnase GFP-  0.0222(/min)
 +
<br>Plac Barnase GFP+  0.0117(/min)
 +
  
 
<!-- -->
 
<!-- -->

Latest revision as of 03:43, 19 September 2015

Translational unit of Barnase

This part is improvement of Part:BBa_I716211. We added a start codon and charactarized it. If you want to know more detail, visit our wiki.
Barnase is a RNase from Bacillus amyloliquefaciens. Its cytotoxity can be neutralized by immunity protein called Barstar (Part:BBa_K1631007).
This coding sequence only contains catalitic domain (not include extracellular translocation signal peptide of Bacillus amyloliquefaciens).


Usage and Biology

We succeeded to control growth rate of E coli.

We induced this parts and [Plac -rbs- GFP -d.term] with 100μM IPTG. [Plac -rbs- GFP -d.term] is negative control. We measured OD600 of 3 samples for each condition.

alt text

We also evaluated the growth rate by slope of next graph.

alt text


Plac GFP- 0.0213(/min)
Plac GFP+ 0.0205(/min)
Plac Barnase GFP- 0.0222(/min)
Plac Barnase GFP+ 0.0117(/min)


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]