Difference between revisions of "Part:BBa K1850012:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | + | ''fim'' was amplified with the native ribosomal binding sites, so that we could maintain the dosing of the ''fim'' subunits. | |
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===Source=== | ===Source=== | ||
− | + | The type I pili structural and transport genes from the ''fim'' operon were amplified from the ''E. coli'' K-12 genome. | |
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===References=== | ===References=== |
Latest revision as of 21:56, 18 September 2015
fim
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 2585
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 868
Illegal AgeI site found at 899 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
fim was amplified with the native ribosomal binding sites, so that we could maintain the dosing of the fim subunits.
Source
The type I pili structural and transport genes from the fim operon were amplified from the E. coli K-12 genome.