Difference between revisions of "Part:BBa K1639010:Design"

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===Design Notes===
 
===Design Notes===
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We inserted two Mlu1 cut sites on each side of miR-373 binding so that other iGEM teams could easily replace it with any other sequence.
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We inserted two '''Mlu1''' cut sites on each side of miR-373 binding so that other iGEM teams could easily replace it with any other sequence.
  
 
===Source===
 
===Source===
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===References===
 
===References===
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Xie Z., Wroblewska L., Prochazka L., Weiss R. ve Benenson Y. 2011. "Multi-Input RNAi-Based Logic Circuit for Identification of Specific Cancer Cells". Science, 333(6047), 1307-1311.

Latest revision as of 18:16, 15 September 2015


mammalian LacI with miR-373 binding site


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1170
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1193
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 562
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 787
    Illegal BsaI.rc site found at 1186


Design Notes

We inserted two Mlu1 cut sites on each side of miR-373 binding so that other iGEM teams could easily replace it with any other sequence.

Source

This protein is mammalian codon optimized version of LacI repressor originally derived from E.coli. It also possess nuclear localization signal (NLS) so that it can act on lac operators.

References

Xie Z., Wroblewska L., Prochazka L., Weiss R. ve Benenson Y. 2011. "Multi-Input RNAi-Based Logic Circuit for Identification of Specific Cancer Cells". Science, 333(6047), 1307-1311.