Difference between revisions of "Part:BBa K1723007:Experience"
 
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https://static.igem.org/mediawiki/2015/4/45/EPF_Lausanne_transistor.png
 
https://static.igem.org/mediawiki/2015/4/45/EPF_Lausanne_transistor.png
  
 
<h3>This picture represent the system we want to build using this promoter: a transistor like element in cell. The complex dCas9-sgRNA will bind on the promoter and activate or inhibit it.</h3>
 
<h3>This picture represent the system we want to build using this promoter: a transistor like element in cell. The complex dCas9-sgRNA will bind on the promoter and activate or inhibit it.</h3>
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<br/><br/><br/>
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<h3> Effects of dCas9-ω BBa_K1723000 on BBa_K1723005 promoter </h3>
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https://static.igem.org/mediawiki/2015/0/09/EPF_Lausanne_RESULTS_EXPERIENCE2.png
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<p>promoter strength was assessed using GFPmut2 reporter.</p>
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<br/>
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<p>This chart present the effects of dCas9-ω (BBa_K1723000) on the PAM rich URS J23117Alt promoter (BBa_K1723005) when provided following sgRNAs:<br/><br/>
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[A_alt] sgRNA X4 (BBa_K1723007), activating<br/>
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[R2_alt] sgRNA X0 (BBa_K1723006), inhibiting<br/>
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[R1_alt] sgRNA X35 (BBa_K1723008), inhibiting<br/> <br/>
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In comparison with:<br/><br/>
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[B_alt] apo-dCas9-ω (=no sgRNA) <br/>
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[Af_alt] apo-dCas9-ω (=no sgRNA), no reporter<br/>
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</p>
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<br/>
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<h3>This chart show the response of the promoter PAM rich URS J23117Alt (BBa_K1723001) in the presence of different complexes sgRNA-dCas9-ω provided by our part collection. Specifically, we see that in presence of the complex BBa_K1723007-dCas9-ω, the promoter PAM rich URS J23117 (BBa_K1723001) is activated. Visit our website for more informations: http://2015.igem.org/Team:EPF_Lausanne</h3>
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===User Reviews===
 
===User Reviews===

Latest revision as of 20:33, 18 September 2015


Applications of BBa_K1723007




EPF_Lausanne_transistor.png

This picture represent the system we want to build using this promoter: a transistor like element in cell. The complex dCas9-sgRNA will bind on the promoter and activate or inhibit it.




Effects of dCas9-ω BBa_K1723000 on BBa_K1723005 promoter

EPF_Lausanne_RESULTS_EXPERIENCE2.png

promoter strength was assessed using GFPmut2 reporter.


This chart present the effects of dCas9-ω (BBa_K1723000) on the PAM rich URS J23117Alt promoter (BBa_K1723005) when provided following sgRNAs:

[A_alt] sgRNA X4 (BBa_K1723007), activating
[R2_alt] sgRNA X0 (BBa_K1723006), inhibiting
[R1_alt] sgRNA X35 (BBa_K1723008), inhibiting

In comparison with:

[B_alt] apo-dCas9-ω (=no sgRNA)
[Af_alt] apo-dCas9-ω (=no sgRNA), no reporter


This chart show the response of the promoter PAM rich URS J23117Alt (BBa_K1723001) in the presence of different complexes sgRNA-dCas9-ω provided by our part collection. Specifically, we see that in presence of the complex BBa_K1723007-dCas9-ω, the promoter PAM rich URS J23117 (BBa_K1723001) is activated. Visit our website for more informations: http://2015.igem.org/Team:EPF_Lausanne




User Reviews

UNIQbf4a942eb1ea6350-partinfo-00000000-QINU UNIQbf4a942eb1ea6350-partinfo-00000001-QINU